Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2999718..3000372 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HED12_RS14965 Protein ID WP_000244781.1
Coordinates 2999965..3000372 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HED12_RS14960 Protein ID WP_000354046.1
Coordinates 2999718..2999984 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS14940 2995806..2997239 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HED12_RS14945 2997284..2997595 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HED12_RS14950 2997759..2998418 + 660 WP_000250274.1 hemolysin III family protein -
HED12_RS14955 2998495..2999475 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
HED12_RS14960 2999718..2999984 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HED12_RS14965 2999965..3000372 + 408 WP_000244781.1 protein YgfX Toxin
HED12_RS14970 3000412..3000933 - 522 WP_001055874.1 flavodoxin FldB -
HED12_RS14975 3001045..3001941 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HED12_RS14980 3001966..3002676 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HED12_RS14985 3002682..3004415 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T162582 WP_000244781.1 NZ_CP056077:2999965-3000372 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T162582 NZ_CP071861:c2180571-2180209 [Enterobacter cloacae]
ATGTGGAACGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAAAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT162582 WP_000354046.1 NZ_CP056077:2999718-2999984 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT162582 NZ_CP071861:c2180221-2179922 [Enterobacter cloacae]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGCATTGCGGCGAAAGTTGAAGTAATGCG
CAGAATTGTTGCGCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCCACGGTGGCGAAAATCGAACTGCCGGGAAACGACCCCCGACTTTCAACGCTCAAACGCTATGTGAGTGCGCTTGGA
GGGGAGCTAAGCATCGATGTGACGTTACCAAACGGGAAACGGATAGCGTTTGAGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References