Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2323618..2324249 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag HED12_RS11575 Protein ID WP_001260301.1
Coordinates 2323618..2323893 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HED12_RS11580 Protein ID WP_000593555.1
Coordinates 2323890..2324249 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS11560 2318622..2319689 + 1068 WP_096964179.1 HlyD family secretion protein -
HED12_RS11565 2319686..2322421 + 2736 WP_000149127.1 ribosome-associated ATPase/putative transporter RbbA -
HED12_RS11570 2322421..2323545 + 1125 WP_001314210.1 ABC transporter permease -
HED12_RS11575 2323618..2323893 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
HED12_RS11580 2323890..2324249 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HED12_RS11585 2324369..2324770 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HED12_RS11590 2324776..2325582 - 807 WP_000173679.1 nickel import ATP-binding protein NikE -
HED12_RS11595 2325579..2326343 - 765 WP_001136232.1 nickel import ATP-binding protein NikD -
HED12_RS11600 2326343..2327176 - 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
HED12_RS11605 2327173..2328117 - 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T162581 WP_001260301.1 NZ_CP056077:2323618-2323893 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T162581 NZ_CP071861:c2103978-2103760 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGTCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT162581 WP_000593555.1 NZ_CP056077:2323890-2324249 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT162581 NZ_CP071861:c2104380-2104006 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References