Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1554498..1554995 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HED12_RS08110 Protein ID WP_001173343.1
Coordinates 1554498..1554671 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HED12_RS08115 Protein ID WP_001312331.1
Coordinates 1554699..1554995 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS08100 1551168..1552625 + 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -
HED12_RS08105 1552862..1554379 + 1518 WP_000003806.1 lysine--tRNA ligase -
HED12_RS08110 1554498..1554671 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HED12_RS08115 1554699..1554995 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HED12_RS08120 1555224..1555496 - 273 WP_000405647.1 N-acetyltransferase -
HED12_RS08125 1555508..1555738 - 231 WP_096939083.1 (4Fe-4S)-binding protein -
HED12_RS08130 1555919..1557550 + 1632 WP_001216466.1 sensor histidine kinase -
HED12_RS08135 1557547..1558266 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T162579 WP_001173343.1 NZ_CP056077:c1554671-1554498 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T162579 NZ_CP071861:1594098-1594331 [Enterobacter cloacae]
CTGGGGGAGACTGTAAAAAAGCAGTTCAGGAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCT
GCATAACCTGCCTGATTGCTACAAAATAAAACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGG
TAACGGTAGTTGTCATCGCTATTGGGAAAAGAGAGAAATTAACCGTGTATCACGATGCTAACAAACGGCTATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT162579 WP_001312331.1 NZ_CP056077:c1554995-1554699 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT162579 NZ_CP071861:1593815-1594057 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGGCGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAGTTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References