Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 715904..716522 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HED12_RS04200 Protein ID WP_001291435.1
Coordinates 716304..716522 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HED12_RS04195 Protein ID WP_000344800.1
Coordinates 715904..716278 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS04185 710994..712187 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HED12_RS04190 712210..715359 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
HED12_RS04195 715904..716278 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HED12_RS04200 716304..716522 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HED12_RS04205 716695..717246 + 552 WP_000102539.1 maltose O-acetyltransferase -
HED12_RS04210 717362..717832 + 471 WP_000136192.1 YlaC family protein -
HED12_RS04215 717996..719546 + 1551 WP_085455481.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HED12_RS04220 719588..719941 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HED12_RS04230 720320..720631 + 312 WP_000409908.1 MGMT family protein -
HED12_RS04235 720662..721234 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162574 WP_001291435.1 NZ_CP056077:716304-716522 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T162574 NZ_CP071861:c122426-122013 [Enterobacter cloacae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCGTTATTGCTCCACGGCCTGGTCGCGGC
ATTTGTTTTACTGATGCCGTGGCCGCTTAGCTATACGCCGCTGTGGCTGTTGTTGCTGTCGTTCGTCGTTTTTGACAGCG
TGCGCAGCCAGCGCCGGATCAATGCCCGCCAGGGTGAGATCAAATTACTGATGGACTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAGTTCCGGCATGATGCTACGGTTACGCCAGGTGGGCGGCGGTAAGTG
CCAGCATCTGTGGCTGGCCGCCGACAGTATGGATATTGCGGAGTGGCGGGAACTGCGCCGGATGCTACTGCAGCAGCAAC
CGACGCAGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT162574 WP_000344800.1 NZ_CP056077:715904-716278 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT162574 NZ_CP071861:c122673-122407 [Enterobacter cloacae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGACGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGAGGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCAGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References