Detailed information of TA system TA162572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 40597..41144
• Replicon: plasmid pMS1170_1
• Accession: NZ_CP056076
• Organism: Escherichia coli strain MS1170

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HED12_RS00235
• Protein ID: WP_139508268.1
• Coordinates: 40597..40878 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: HED12_RS00240
• Protein ID: WP_000079941.1
• Coordinates: 40875..41144 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HED12_RS00200	35602..35844	+	243	WP_000844627.1	transposase	-
HED12_RS00205	35876..36526	-	651	WP_000164043.1	tetracycline resistance transcriptional repressor TetR(A)	-
HED12_RS00210	36632..37831	+	1200	WP_000804064.1	tetracycline efflux MFS transporter Tet(A)	-
HED12_RS00215	37863..38723	-	861	WP_000493383.1	EamA family transporter	-
HED12_RS00220	38732..39346	-	615	Protein_43	helix-turn-helix domain-containing protein	-
HED12_RS00225	39308..39466	+	159	WP_197711125.1	MerR family transcriptional regulator	-
HED12_RS00230	39703..40551	-	849	WP_001058005.1	3'-5' exonuclease	-
HED12_RS00235	40597..40878	-	282	WP_139508268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HED12_RS00240	40875..41144	-	270	WP_000079941.1	hypothetical protein	Antitoxin
HED12_RS00245	41199..41357	-	159	WP_000841008.1	hypothetical protein	-
HED12_RS00250	42050..42914	-	865	Protein_49	incFII family plasmid replication initiator RepA	-
HED12_RS00255	43624..44265	+	642	WP_077250484.1	transcription termination factor NusG	-
HED12_RS00260	44407..45072	+	666	WP_029391921.1	hypothetical protein	-
HED12_RS00265	45130..45591	+	462	WP_174843148.1	PilI type IV pilus biogenesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / aac(3)-VIa / qacE / sul1 / tet(A) / blaCMY-2	htpB	1..122112	122112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10921.62 Da
• Isoelectric Point: 8.4615

>T162572 WP_139508268.1 NZ_CP056076:c40878-40597 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T162572 NZ_CP071860:c1025465-1025196 [Vibrio sp. SCSIO 43135]
ATGAGTAGTAGTCAACGTTTACTATCGTGGACGGATGACGCTTGGGGTGATTATCTGTATTGGCAAACCCAAGATAAGAA
AGCGCTTAAGCGTATCAACAAACTCATCAACGATGTTAAGCGTTCTCCTTTTGAGGGTATTGGTAAACCCGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGCCGCATTGACGATACTAATAGGCTTGTTTACGCAGTCGATGACCAAGCAATA
ACAATAATTTCATGTCGCTACCACTACTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT162572 WP_000079941.1 NZ_CP056076:c41144-40875 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT162572 NZ_CP071860:c1025712-1025458 [Vibrio sp. SCSIO 43135]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTAGACGGTGTAGTTAATGATGCAGACACGAC
AGTTATTACACGTCGTGACTCTGAAGATGCTGTGGTAATGTCTTTGGACTACTACAACAGTCTTATGGAGACAGTGCACT
TACTACGCTCTCCTCAAAATGCTGAACACCTAAACCGGTCAATAGCACAGTACCGTGCCGGTAAAACAACCGCACGAGAG
TTAATTGATGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5