Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 187..709 Replicon plasmid pEC50-2
Accession NZ_CP056075
Organism Escherichia coli strain EC50

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag HUS69_RS23015 Protein ID WP_000220561.1
Coordinates 187..468 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HUS69_RS23020 Protein ID WP_000121743.1
Coordinates 458..709 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS69_RS23015 (187) 187..468 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUS69_RS23020 (458) 458..709 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HUS69_RS23025 (1944) 1944..2783 + 840 WP_024129959.1 replication initiation protein -
HUS69_RS23030 (2804) 2804..3646 + 843 WP_073446731.1 replication initiation protein -
HUS69_RS23035 (3650) 3650..4096 + 447 WP_001074384.1 hypothetical protein -
HUS69_RS23040 (4233) 4233..4586 + 354 WP_024129958.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR / oqxA / oqxB / aph(3')-IIa - 1..39140 39140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T162571 WP_000220561.1 NZ_CP056075:c468-187 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T162571 NZ_CP071857:c1494783-1494487 [Vibrio sp. SCSIO 43145]
ATGCAAAAGAACAAATACAAACTGAGTAAATTAGCTCAGACTCATTTACGGAAAATCAAAAGCTATACTGTTAACAACTT
CTCCGAGACCCAGTGGCGAAGCTATAAAGATACGTTACTAACTGGTTTTCAAATGCTTGCTGATAATCCAGCTGTTGGTC
GCAGTTGTGATGACATATACCCAAGCGGGTTCTATTTCCCTGTGGGCAAGCATACCGCTTATTTTACAAAAGAGGATGGC
TTCATTTTAGTTGTAGCTATACTCGGTCAATCACAGCTTCCTCAGAACCACTTATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT162571 WP_000121743.1 NZ_CP056075:c709-458 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT162571 NZ_CP071857:c1495032-1494790 [Vibrio sp. SCSIO 43145]
ATGCACACATTAACAGCAAATGATGCCAAACGTAATTTTGGTGAGCTACTTCTAAGCGCCCAACGTGAGCCTGTGAAGAT
TAGCAAAAACAGTAAAGATGCCGTCGTTGTAATGTCTATCAAGGACTATGAAGAACTAGAAGCAATGAAAACCGATTACC
TTAAGCACTGTTTCGAGTCAGCGAAACAAGACTTAGCTCAAGGAAATGTGGTTGATGGTGAAGACTTTTTAAACGCCTTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References