Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4226795..4227292 Replicon chromosome
Accession NZ_CP056073
Organism Escherichia coli strain EC50

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUS69_RS20370 Protein ID WP_001173343.1
Coordinates 4226795..4226968 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUS69_RS20375 Protein ID WP_000398619.1
Coordinates 4226996..4227292 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS69_RS20360 (4223465) 4223465..4224922 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HUS69_RS20365 (4225159) 4225159..4226676 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUS69_RS20370 (4226795) 4226795..4226968 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUS69_RS20375 (4226996) 4226996..4227292 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUS69_RS20380 (4227519) 4227519..4227791 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HUS69_RS20385 (4227803) 4227803..4228033 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUS69_RS20390 (4228214) 4228214..4229845 + 1632 WP_001216477.1 sensor histidine kinase -
HUS69_RS20395 (4229842) 4229842..4230561 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T162567 WP_001173343.1 NZ_CP056073:c4226968-4226795 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T162567 NZ_CP071857:c1471258-1470980 [Vibrio sp. SCSIO 43145]
ATGATTTTTTGGGAAGAAGAGTCTCTCAACGATCGTGAAAAGATCTTCGAATTTCTTTACGACTTCAACCCAGAGGCTGC
TGAAAAAACCGATGAAGTTATCGAAGCCAAAGTGGAGAACCTTCTAGAACAACCTCATATGGGAGTCCAACGTGACGGAA
TTAGAGGGCGATTGCTCATTATTCCTGAGATATCAATGATTGTTTCATACTGGATTGAAGGCGATATCATTCGCATTATG
CGTGTACTACATCAAAAACAAAAATTCCCTGCGAGCTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT162567 WP_000398619.1 NZ_CP056073:c4227292-4226996 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT162567 NZ_CP071857:c1471539-1471255 [Vibrio sp. SCSIO 43145]
ATGGACACCAGAATTCAGTTTCGTGTTGATGAAGAAACGAAGCGACTAGCTCAACAAATGGCAGAAAGCCAAGGCAGAAC
ACTAAGCGACGCTTGTCGTGAACTGACTGAACAACTAGCTGACCAACAGAGAAAGACTTTATCTCACGATGCATGGCTAA
CTGAACAAGTTAACTTGGCTTTTGAGAAGTTTGACTTAGGAAAAGCCAACTTTGTTGATCACGAATCAGCTAAATCACGT
ATGGCTGAGCGCAAAGCAAAAATCCGTAACCGAGGCAAAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References