Detailed information of TA system TA162566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4062605..4064288
• Replicon: chromosome
• Accession: NZ_CP056073
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: I2UPR9
• Locus tag: HUS69_RS19565
• Protein ID: WP_000361917.1
• Coordinates: 4063371..4064288 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: HUS69_RS19560
• Protein ID: WP_024243514.1
• Coordinates: 4062605..4063378 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS69_RS19545 (4058461)	4058461..4060545	+	2085	WP_001193073.1	protease Lon-related BREX system protein BrxL	-
HUS69_RS19550 (4060664)	4060664..4062013	+	1350	WP_024243516.1	phospholipase D-like domain-containing protein	-
HUS69_RS19555 (4062189)	4062189..4062517	-	329	Protein_3876	IS3 family transposase	-
HUS69_RS19560 (4062605)	4062605..4063378	+	774	WP_024243514.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HUS69_RS19565 (4063371)	4063371..4064288	+	918	WP_000361917.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HUS69_RS19570 (4064582)	4064582..4064749	+	168	WP_001356018.1	hypothetical protein	-
HUS69_RS19575 (4064802)	4064802..4065464	-	663	WP_000357928.1	hypothetical protein	-
HUS69_RS19580 (4065537)	4065537..4066178	-	642	Protein_3881	integrase arm-type DNA-binding domain-containing protein	-
HUS69_RS19585 (4066544)	4066544..4066987	-	444	Protein_3882	HTH domain-containing protein	-
HUS69_RS19590 (4067190)	4067190..4067735	-	546	Protein_3883	GTPase	-
HUS69_RS19595 (4067734)	4067734..4068002	+	269	Protein_3884	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	papC / papD	4062605..4082385	19780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34963.04 Da
• Isoelectric Point: 4.7716

>T162566 WP_000361917.1 NZ_CP056073:4063371-4064288 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELEAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T162566 NZ_CP071857:c1445741-1445463 [Vibrio sp. SCSIO 43145]
ATGATTTTTTGGGAAGAAGAGTCTCTCAACGATCGTGAAAAGATCTTCGAATTTCTTTACGACTTCAACCCAGAGGCTGC
TGAAAAAACCGATGAAGTTATCGAAGCCAAAGTGGAGAACCTTCTAGAACAACCTCATATGGGAGTCCAACGTGACGGAA
TTAGAGGGCGATTGCTCATTATTCCTGAGATATCAATGATTGTTTCATACTGGATTGAAGGCGATATCATTCGCATTATG
CGTGTACTACATCAAAAACAAAAATTCCCTGCGAGCTAG

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29319.73 Da
• Isoelectric Point: 9.9889

>AT162566 WP_024243514.1 NZ_CP056073:4062605-4063378 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPGVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT162566 NZ_CP071857:c1446022-1445738 [Vibrio sp. SCSIO 43145]
ATGGACACCAGAATTCAGTTTCGTGTTGATGAAGAAACGAAGCGACTAGCTCAACAAATGGCAGAAAGCCAAGGCAGAAC
ACTAAGCGACGCTTGTCGTGAACTGACTGAACAACTAGCTGACCAACAGAGAAAGACTTTATCTCACGATGCATGGCTAA
CTGAACAAGTTAACTTGGCTTTTGAGAAGTTTGACTTAGGAAAAGCCAACTTTGTTGATCACGAATCAGCTAAATCACGT
ATGGCTGAGCGCAAAGCAAAAATCCGTAACCGAGGCAAAGTATGA

Structures

Toxin

• AlphaFold DB: A0A0H0PS08