Detailed information of TA system TA162563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3517054..3517891
• Replicon: chromosome
• Accession: NZ_CP056073
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUS69_RS16990
• Protein ID: WP_000227784.1
• Coordinates: 3517349..3517891 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUS69_RS16985
• Protein ID: WP_001297137.1
• Coordinates: 3517054..3517365 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS69_RS16960 (3512074)	3512074..3513021	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
HUS69_RS16965 (3513043)	3513043..3515034	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUS69_RS16970 (3515024)	3515024..3515638	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUS69_RS16975 (3515638)	3515638..3515967	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUS69_RS16980 (3515979)	3515979..3516869	+	891	WP_000971336.1	heme o synthase	-
HUS69_RS16985 (3517054)	3517054..3517365	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUS69_RS16990 (3517349)	3517349..3517891	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUS69_RS16995 (3517947)	3517947..3518882	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
HUS69_RS17000 (3519290)	3519290..3520654	+	1365	WP_001000978.1	MFS transporter	-
HUS69_RS17005 (3520782)	3520782..3521273	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUS69_RS17010 (3521441)	3521441..3522352	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T162563 WP_000227784.1 NZ_CP056073:3517349-3517891 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T162563 NZ_CP071855:c1887866-1887564 [Vibrio sp. SCSIO 43153]
ATGGCTGAAATAATCTGGACTGAGCCAGCGTTATCTGACCTCAACGATATCGCTGAATACATCGCAATTGAAAATATCGT
AGCTGCAAAGCAGTTAGTGCAAACGATCTTCTCCAGAGTCGAACGCCTACAAATTTTCCCAGAGTCAGGCCGTATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAAATTTGCGTAAGTTCTTGTTGAGTAAGCAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT162563 WP_001297137.1 NZ_CP056073:3517054-3517365 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT162563 NZ_CP071855:c1888111-1887854 [Vibrio sp. SCSIO 43153]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAGCATGGTAAGCCATCCGCGTATCTCGTTGATGTTGATGACTACGAGTTTATGCAAAATCGTTTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGACGGTAAAGTGGTAAGTCATGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3