Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3482973..3483591 Replicon chromosome
Accession NZ_CP056073
Organism Escherichia coli strain EC50

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUS69_RS16820 Protein ID WP_001291435.1
Coordinates 3483373..3483591 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUS69_RS16815 Protein ID WP_000344800.1
Coordinates 3482973..3483347 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS69_RS16805 (3478062) 3478062..3479255 + 1194 WP_089046497.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUS69_RS16810 (3479278) 3479278..3482427 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUS69_RS16815 (3482973) 3482973..3483347 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUS69_RS16820 (3483373) 3483373..3483591 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUS69_RS16825 (3483763) 3483763..3484314 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUS69_RS16830 (3484430) 3484430..3484900 + 471 WP_000136192.1 YlaC family protein -
HUS69_RS16835 (3485064) 3485064..3486614 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUS69_RS16840 (3486656) 3486656..3487009 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUS69_RS16850 (3487388) 3487388..3487699 + 312 WP_000409911.1 MGMT family protein -
HUS69_RS16855 (3487730) 3487730..3488302 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162562 WP_001291435.1 NZ_CP056073:3483373-3483591 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T162562 NZ_CP071852:2198266-2198544 [Vibrio sp. SCSIO 43155]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTATCTCTTATTGGGTCGAGGGCGATATCATCAGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCTACGAATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT162562 WP_000344800.1 NZ_CP056073:3482973-3483347 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT162562 NZ_CP071852:2197985-2198269 [Vibrio sp. SCSIO 43155]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGAAC
TCTAAGTGATGCTTGCCGCGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCTAGGTAAGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References