Detailed information of TA system TA162561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3087391..3088096
• Replicon: chromosome
• Accession: NZ_CP056073
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HUS69_RS14895
• Protein ID: WP_000539521.1
• Coordinates: 3087391..3087777 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUS69_RS14900
• Protein ID: WP_001280945.1
• Coordinates: 3087767..3088096 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS69_RS14875 (3083395)	3083395..3084021	+	627	WP_089622771.1	glutathione S-transferase GstB	-
HUS69_RS14880 (3084018)	3084018..3085133	-	1116	WP_186240721.1	aldose sugar dehydrogenase YliI	-
HUS69_RS14885 (3085244)	3085244..3085627	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HUS69_RS14890 (3085840)	3085840..3087165	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUS69_RS14895 (3087391)	3087391..3087777	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS69_RS14900 (3087767)	3087767..3088096	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUS69_RS14905 (3088166)	3088166..3089493	-	1328	Protein_2961	diguanylate cyclase	-
HUS69_RS14910 (3089501)	3089501..3091848	-	2348	Protein_2962	EAL domain-containing protein	-
HUS69_RS14915 (3092026)	3092026..3092937	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T162561 WP_000539521.1 NZ_CP056073:3087391-3087777 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T162561 NZ_CP071852:2163142-2163420 [Vibrio sp. SCSIO 43155]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTTAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTGTCTTACTGGCTCGAGGGCGATATCATCCGAGTTATG
CGTGTACTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT162561 WP_001280945.1 NZ_CP056073:3087767-3088096 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT162561 NZ_CP071852:2162861-2163145 [Vibrio sp. SCSIO 43155]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTAAGTGATGCTTGCCGTGAACTTACAGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTAAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6