162545

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 2067..2589 Replicon plasmid pEC31-3
Accession NZ_CP056039
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag HUW39_RS26015 Protein ID WP_000220561.1
Coordinates 2067..2348 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HUW39_RS26020 Protein ID WP_000121743.1
Coordinates 2338..2589 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS25995 (70) 70..312 - 243 WP_000008708.1 hypothetical protein -
HUW39_RS26000 (302) 302..571 - 270 WP_001006718.1 hypothetical protein -
HUW39_RS26005 (580) 580..1128 - 549 WP_001178641.1 hypothetical protein -
HUW39_RS26010 (1202) 1202..1717 - 516 WP_001025387.1 J domain-containing protein -
HUW39_RS26015 (2067) 2067..2348 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUW39_RS26020 (2338) 2338..2589 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HUW39_RS26025 (3824) 3824..4663 + 840 WP_001352874.1 replication initiation protein -
HUW39_RS26030 (4684) 4684..5526 + 843 WP_000435064.1 replication initiation protein -
HUW39_RS26035 (5530) 5530..5976 + 447 WP_001074384.1 hypothetical protein -
HUW39_RS26040 (6113) 6113..6469 + 357 WP_001714234.1 DNA distortion polypeptide 3 -
HUW39_RS26045 (6490) 6490..7350 - 861 WP_063122221.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid floR - 1..29451 29451


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T162545 WP_000220561.1 NZ_CP056039:c2348-2067 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T162545 NZ_CP071848:c623934-623782 [Vibrio sp. SCSIO 43136]
ATGAAGCAGCTACTTGCATATATGAATGGTGAACTTGTAGGGGCTCTAGTCAAGCGAGCGAACGGTGCGCATTCATTTAC
TTATGACGCCAGCTGGCTGAGCAATGAAAAGGCTCGACCTCTATCGCTAAAACTGCAAGTGCCGATCATCTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT162545 WP_000121743.1 NZ_CP056039:c2589-2338 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT162545 NZ_CP071848:c624182-623937 [Vibrio sp. SCSIO 43136]
ATGATATATAGCCCAAAACAATTGGCTGACTTCTTACTGCTAGTTCGCCAAAAAAACCGTTGGACTCAAGCAGAGCTAGC
CAAAAAAGTGGGTATCAAGCAGGCAACGATCTCTAATTTTGAAAACAACCCTGATAAAACCACGCTATCAACCATGTTCA
AGATCATCCAAGCACTTGAACTAAAGCTAGAAGTTAAAGAGCCATTTGAAGTCAAAGAGCCTAGTGTTGATGAGGAAGAC
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References