Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 99220..99863 Replicon plasmid pEC31-1
Accession NZ_CP056037
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HUW39_RS25495 Protein ID WP_001034046.1
Coordinates 99447..99863 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HUW39_RS25490 Protein ID WP_001261278.1
Coordinates 99220..99450 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS25460 (HUW39_25455) 94731..95036 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
HUW39_RS25465 (HUW39_25460) 95038..95256 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HUW39_RS25470 (HUW39_25465) 95823..96335 + 513 WP_000151784.1 hypothetical protein -
HUW39_RS25475 (HUW39_25470) 96369..97502 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HUW39_RS25480 (HUW39_25475) 97669..98442 - 774 WP_000905949.1 hypothetical protein -
HUW39_RS25485 (HUW39_25480) 98455..98955 - 501 WP_000528931.1 HEPN family nuclease -
HUW39_RS25490 (HUW39_25485) 99220..99450 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUW39_RS25495 (HUW39_25490) 99447..99863 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HUW39_RS25500 (HUW39_25495) 99908..103702 - 3795 WP_001144732.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / mph(A) / aac(3)-IId / aadA5 / blaCTX-M-55 / blaTEM-135 / fosA3 / sitABCD iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA 1..122881 122881


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T162544 WP_001034046.1 NZ_CP056037:99447-99863 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T162544 NZ_CP071848:c374225-373878 [Vibrio sp. SCSIO 43136]
ATGTGGAACATCGAGACTAGAGCTAGATTTGATGATTGGTACTGGTCACTAACAGACCCTGACCGTATATCAATCCAAGC
TTCCTTATTAGTCTTAGCTGAAAAGGGAGCGCAACTGGGTCGTCCTCATGCTGACTCACTGCACGGCTCAAAGCACTCCA
ATATGAAAGAACTCAGAGTCCAAAGTCAGGGAAGGCCAATAAGAATATTTTTTGCTTTTGACCCTGAGCGAACAGGTATT
GTCTTATGCGGCGGAGATAAATCGGGAGACAAGCGTTTCTATAGAACGATGATACCGATTGCTGATGCTGAATATGACAA
GCATCTAGAGGAGTTAAAAAATGGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT162544 WP_001261278.1 NZ_CP056037:99220-99450 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT162544 NZ_CP071848:c373885-373574 [Vibrio sp. SCSIO 43136]
ATGGCTAAACAAAGCAAACCTCTAAGCGAATTACTCGCCAAAGAGAAACCTGAAGTTCTAGAGCAAGCTCGACTAAAAGC
TGATGAAATGCTGTTAGACTTACGCTTGGCGGAAATACGAACACTAACAAACCAATCCCAAGTTCAAATAGCCCAAGCGA
TGGGTGTCACTCAACCGACCATCGCCAGCCTTGAAAAGAAAGGAAAAGACGTACGCCTCATGTCGTTAAAGCGCTACGTT
GAGGCTGCTGGTTGTAAACTTAGAATCGATATTGAAATGCCAGATGGCAGTAGCCACGAGTTACCAATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References