Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 94731..95256 | Replicon | plasmid pEC31-1 |
| Accession | NZ_CP056037 | ||
| Organism | Escherichia coli strain EC31 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | V0SSI5 |
| Locus tag | HUW39_RS25460 | Protein ID | WP_001159868.1 |
| Coordinates | 94731..95036 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | S1PPD8 |
| Locus tag | HUW39_RS25465 | Protein ID | WP_000813634.1 |
| Coordinates | 95038..95256 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUW39_RS25445 (HUW39_25440) | 90641..91807 | - | 1167 | WP_000772446.1 | plasmid-partitioning protein SopA | - |
| HUW39_RS25450 (HUW39_25445) | 92395..93150 | - | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| HUW39_RS25455 (HUW39_25450) | 93924..94730 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| HUW39_RS25460 (HUW39_25455) | 94731..95036 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| HUW39_RS25465 (HUW39_25460) | 95038..95256 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| HUW39_RS25470 (HUW39_25465) | 95823..96335 | + | 513 | WP_000151784.1 | hypothetical protein | - |
| HUW39_RS25475 (HUW39_25470) | 96369..97502 | - | 1134 | WP_001542067.1 | DUF3800 domain-containing protein | - |
| HUW39_RS25480 (HUW39_25475) | 97669..98442 | - | 774 | WP_000905949.1 | hypothetical protein | - |
| HUW39_RS25485 (HUW39_25480) | 98455..98955 | - | 501 | WP_000528931.1 | HEPN family nuclease | - |
| HUW39_RS25490 (HUW39_25485) | 99220..99450 | + | 231 | WP_001261278.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| HUW39_RS25495 (HUW39_25490) | 99447..99863 | + | 417 | WP_001034046.1 | type II toxin-antitoxin system VapC family toxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(A) / mph(A) / aac(3)-IId / aadA5 / blaCTX-M-55 / blaTEM-135 / fosA3 / sitABCD | iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA | 1..122881 | 122881 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11706.51 Da Isoelectric Point: 6.4674
>T162543 WP_001159868.1 NZ_CP056037:c95036-94731 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T162543 NZ_CP071845:c1428331-1428035 [Vibrio sp. SCSIO 43139]
ATGCAAAAGAACAAATACAAACTGAGTAAATTAGCTCAGACTCATTTACGGAAAATCAAAAGCTATACTGTTAACAACTT
CTCCGAGACCCAGTGGCGAAGCTATAAAGATACGTTACTAACTGGTTTTCAAATGCTTGCTGATAATCCAGCTGTTGGTC
GCAGTTGTGATGACATATACCCAAGCGGGTTCTATTTCCCTGTGGGCAAGCATACCGCTTATTTTACAAAAGAGGATGGC
TTCATTTTAGTTGTAGCTATACTCGGTCAATCACAGCTTCCTCAGAACCACTTATAA
ATGCAAAAGAACAAATACAAACTGAGTAAATTAGCTCAGACTCATTTACGGAAAATCAAAAGCTATACTGTTAACAACTT
CTCCGAGACCCAGTGGCGAAGCTATAAAGATACGTTACTAACTGGTTTTCAAATGCTTGCTGATAATCCAGCTGTTGGTC
GCAGTTGTGATGACATATACCCAAGCGGGTTCTATTTCCCTGTGGGCAAGCATACCGCTTATTTTACAAAAGAGGATGGC
TTCATTTTAGTTGTAGCTATACTCGGTCAATCACAGCTTCCTCAGAACCACTTATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT162543 WP_000813634.1 NZ_CP056037:c95256-95038 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT162543 NZ_CP071845:c1428580-1428338 [Vibrio sp. SCSIO 43139]
ATGCACACATTAACAGCAAATGATGCCAAACGTAATTTTGGTGAGCTACTTCTAAGCGCCCAACGTGAGCCTGTGAAGAT
TAGCAAAAACAGTAAAGATGCCGTCGTTGTAATGTCTATCAAGGACTATGAAGAACTAGAAGCAATGAAAACCGATTACC
TTAAGCACTGTTTCGAGTCAGCGAAACAAGACTTAGCTCAAGGAAATGTGGTTGATGGTGAAGACTTTTTAAACGCCTTA
TAA
ATGCACACATTAACAGCAAATGATGCCAAACGTAATTTTGGTGAGCTACTTCTAAGCGCCCAACGTGAGCCTGTGAAGAT
TAGCAAAAACAGTAAAGATGCCGTCGTTGTAATGTCTATCAAGGACTATGAAGAACTAGAAGCAATGAAAACCGATTACC
TTAAGCACTGTTTCGAGTCAGCGAAACAAGACTTAGCTCAAGGAAATGTGGTTGATGGTGAAGACTTTTTAAACGCCTTA
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|