Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4747327..4747824 Replicon chromosome
Accession NZ_CP056036
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUW39_RS22870 Protein ID WP_001173343.1
Coordinates 4747327..4747500 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUW39_RS22875 Protein ID WP_000398619.1
Coordinates 4747528..4747824 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS22860 (4743998) 4743998..4745455 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
HUW39_RS22865 (4745692) 4745692..4747209 + 1518 WP_000003806.1 lysine--tRNA ligase -
HUW39_RS22870 (4747327) 4747327..4747500 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUW39_RS22875 (4747528) 4747528..4747824 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUW39_RS22880 (4748053) 4748053..4748325 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUW39_RS22885 (4748337) 4748337..4748567 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
HUW39_RS22890 (4748748) 4748748..4750379 + 1632 WP_001216477.1 sensor histidine kinase -
HUW39_RS22895 (4750376) 4750376..4751095 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T162542 WP_001173343.1 NZ_CP056036:c4747500-4747327 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T162542 NZ_CP071845:1424050-1424319 [Vibrio sp. SCSIO 43139]
ATGAGTAGTAGTCAACGTTTACTATCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATGTAAAGCGCTCCCCATTTGAGGGTATCGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTGTTGATGACACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATCTCGTGTCGTTACCACTACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT162542 WP_000398619.1 NZ_CP056036:c4747824-4747528 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT162542 NZ_CP071845:1423803-1424057 [Vibrio sp. SCSIO 43139]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTGGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAAGATGCTGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACCGTTCACT
TACTACGCTCTCCTCAAAACGCTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAACACGAGAG
TTAATTGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References