162533

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3867036..3867654 Replicon chromosome
Accession NZ_CP056036
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUW39_RS18705 Protein ID WP_001291435.1
Coordinates 3867436..3867654 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUW39_RS18700 Protein ID WP_000344800.1
Coordinates 3867036..3867410 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS18690 (3862125) 3862125..3863318 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUW39_RS18695 (3863341) 3863341..3866490 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
HUW39_RS18700 (3867036) 3867036..3867410 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUW39_RS18705 (3867436) 3867436..3867654 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUW39_RS18710 (3867826) 3867826..3868377 + 552 WP_000102559.1 maltose O-acetyltransferase -
HUW39_RS18715 (3868493) 3868493..3868963 + 471 WP_000136192.1 YlaC family protein -
HUW39_RS18720 (3869127) 3869127..3870677 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUW39_RS18725 (3870719) 3870719..3871072 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUW39_RS18735 (3871451) 3871451..3871762 + 312 WP_000409911.1 MGMT family protein -
HUW39_RS18740 (3871793) 3871793..3872365 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162533 WP_001291435.1 NZ_CP056036:3867436-3867654 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T162533 NZ_CP071844:72912-73229 [Vibrio sp. SCSIO 43009]
ATGCACGTAATTAAACGAGAGCCTTTTGAAACGGCAAAGCGACTATATCCGCAATGTGCCGAGGCACTAGACTCAACTTT
CAAAACGCTAAAAGCAACAAAAGCAAAAAGACCAGAACAACTCAAACAACTTTTTCCGTCATTGGATAACTTTAAATACG
TAGACAAGTGGTATGTCATCGACATTGGCGGCGGTTCATTAAGGTTAATCGCCTTTATCGAATTTACAGGTGGGAAATGC
TTCGTTAAATACATCGTTACCCACGCAGAATACGACAAGATAGCAGACCAACACAGAACAAAGGGGAAAAGGGGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT162533 WP_000344800.1 NZ_CP056036:3867036-3867410 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT162533 NZ_CP071844:73232-73645 [Vibrio sp. SCSIO 43009]
ATGAACATTCAAGCTCTAAAAACCCTATCAAATGAAATTCACACAGCGATGCCTTGGTTACAAGGCATCCGTGACCATGA
GCAATATTTAGAGCTTATGGAACTCATGGATACGCTAGTAGAAGACTACGAGGAAAACCATGTCTTGATTGACTTGCTAT
TCCCTGTACTTGAACGCTACGAGGAAGAATCAGAACACTTCCAAGAGTTTAACGCGCACATTGATAGCCTAGATACTGGT
ATCGCCACTCTTCGCGTTCTCATCGACCAATACCGCCTAACTCTAAGTGATTTACCTGAGATTGGTGGCAAGTCGCTAGT
ATCGCTAATCCTAAGCGGTAAACGCCAACTAACCGTTGGACACATTAAAGCGCTATCGGAACGTTTCGGCGTACCAAAGC
ATATGTTTATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References