Detailed information of TA system TA162532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3378183..3378888
• Replicon: chromosome
• Accession: NZ_CP056036
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HUW39_RS16295
• Protein ID: WP_000539521.1
• Coordinates: 3378183..3378569 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUW39_RS16300
• Protein ID: WP_001280945.1
• Coordinates: 3378559..3378888 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW39_RS16275 (3374187)	3374187..3374813	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HUW39_RS16280 (3374810)	3374810..3375925	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
HUW39_RS16285 (3376036)	3376036..3376419	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HUW39_RS16290 (3376632)	3376632..3377957	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUW39_RS16295 (3378183)	3378183..3378569	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUW39_RS16300 (3378559)	3378559..3378888	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUW39_RS16305 (3378958)	3378958..3380286	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
HUW39_RS16310 (3380294)	3380294..3382642	-	2349	WP_023148675.1	EAL domain-containing protein	-
HUW39_RS16315 (3382820)	3382820..3383731	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T162532 WP_000539521.1 NZ_CP056036:3378183-3378569 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T162532 NZ_CP071844:24415-24702 [Vibrio sp. SCSIO 43009]
ATGGAATTCAAAACTCAATATTTAGAAGATTTTTATGTAGATGATATTTCGCATAAAAAGATCCCTAGCAATTTAGAAGA
CGTACTGTACAGAAAGCTAGAAATGCTTGATGCAGCAACAGACTTTAGGGATTTGCTGGCACCACCAAATAACAGATTTG
AACGACTAAGCGGCAAGCTTAGTGGATATTGCAGTATCAGAGTTAATAAACAGTATCGTCTCGTCTTTAAGTTTGACAAT
GGAGAGGTTACTGATACCTATTTAGACCCGCACACTTATCGAGCTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT162532 WP_001280945.1 NZ_CP056036:3378559-3378888 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT162532 NZ_CP071844:24801-25130 [Vibrio sp. SCSIO 43009]
ATGAGACGCACAAAGCGTAAGCCAGTAACAGTCGGCGAAATGCTAAAAGTTGAGTTTCTTGAGCCGCTGTCCATAACAAA
TTCTACATTAGCCGAAGCTATGGGCGTTCACCGTAACACCATTAGCAATATTATAAATGGCAAAACCACATTAACATCGG
AATTAGCAACCAAATTAGCCGTAGCGCTTGGAAATACACCTGAGTTTTGGTTGAACATTCAACACGCAGTTGATGTGTGG
GAAAGTAACAACAGCTTTGAAGATACCAAACTAAGTGTTAAGCCTTTGGTAAAGTTTGATAGCTCTATGTCAGATTTCGC
AATGGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6