Detailed information of TA system TA162531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3097242..3098026
• Replicon: chromosome
• Accession: NZ_CP056036
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HUW39_RS14915
• Protein ID: WP_000613626.1
• Coordinates: 3097532..3098026 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: HUW39_RS14910
• Protein ID: WP_001110447.1
• Coordinates: 3097242..3097535 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW39_RS14900 (3092392)	3092392..3093351	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
HUW39_RS14905 (3093924)	3093924..3097109	+	3186	WP_000827427.1	ribonuclease E	-
HUW39_RS14910 (3097242)	3097242..3097535	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
HUW39_RS14915 (3097532)	3097532..3098026	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HUW39_RS14920 (3098121)	3098121..3099074	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
HUW39_RS14925 (3099086)	3099086..3100729	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
HUW39_RS14930 (3100795)	3100795..3101736	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HUW39_RS14935 (3101736)	3101736..3102833	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T162531 WP_000613626.1 NZ_CP056036:3097532-3098026 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T162531 NZ_CP071842:c1347133-1346843 [Vibrio sp. SCSIO 43009]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCACTCAAAGAGTGGAAAAAGCTTGGCTCTACTCTGCAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCACATGTTCCAGCTTCAAAACTTTCTGGAGCTGACAACATGTATAAAATTA
AGTTGCGTCAATCGGGCTACCGTCTCGTCTACAAAGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCAGTCGGAAAA
CGAGAACGTAGCGATGTTTACCGTAAAGCCATGAAAAGGTTAGATGATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT162531 WP_001110447.1 NZ_CP056036:3097242-3097535 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT162531 NZ_CP071842:c1347371-1347123 [Vibrio sp. SCSIO 43009]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACCGAATTGAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTATGGCGAACCTGTTGCAGTATTAAACCGAAATGAGCCAGCATTTTATTGCGTACCTGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTAGCTATCGCTAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6