Detailed information of TA system TA162530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2747138..2747776
• Replicon: chromosome
• Accession: NZ_CP056036
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: HUW39_RS13115
• Protein ID: WP_000813797.1
• Coordinates: 2747600..2747776 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUW39_RS13110
• Protein ID: WP_076611057.1
• Coordinates: 2747138..2747554 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW39_RS13090 (2742290)	2742290..2743231	-	942	WP_001251335.1	ABC transporter permease	-
HUW39_RS13095 (2743232)	2743232..2744245	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
HUW39_RS13100 (2744263)	2744263..2745408	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
HUW39_RS13105 (2745653)	2745653..2747059	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
HUW39_RS13110 (2747138)	2747138..2747554	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUW39_RS13115 (2747600)	2747600..2747776	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUW39_RS13120 (2747998)	2747998..2748228	+	231	WP_000494244.1	YncJ family protein	-
HUW39_RS13125 (2748320)	2748320..2750281	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUW39_RS13130 (2750354)	2750354..2750890	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
HUW39_RS13135 (2750982)	2750982..2752154	+	1173	WP_001236223.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T162530 WP_000813797.1 NZ_CP056036:c2747776-2747600 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T162530 NZ_CP071840:c1434823-1434545 [Vibrio sp. SCSIO 43097]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTTAACCCTGATGCGGC
AGAAAAAACTGACAACCTTATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTACTGGGTCGAGGGCGATATCATCCGAGTTATG
CGTGTACTCCACCAAAAACAAAAATTTCCTACAGATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT162530 WP_076611057.1 NZ_CP056036:c2747554-2747138 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT162530 NZ_CP071840:c1435104-1434820 [Vibrio sp. SCSIO 43097]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTAAGTGATGCTTGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCGTTGAGCACCAAACTGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A829J715