Detailed information of TA system TA162528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2647405..2648993
• Replicon: chromosome
• Accession: NZ_CP056036
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: HUW39_RS12670
• Protein ID: WP_001125415.1
• Coordinates: 2647671..2648993 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUW39_RS12665
• Protein ID: WP_001296726.1
• Coordinates: 2647405..2647671 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW39_RS12665 (2647405)	2647405..2647671	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUW39_RS12670 (2647671)	2647671..2648993	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HUW39_RS12675 (2649391)	2649391..2650290	-	900	WP_001057776.1	LysR family transcriptional regulator	-
HUW39_RS12680 (2650430)	2650430..2650738	+	309	WP_001221046.1	hypothetical protein	-
HUW39_RS12685 (2650747)	2650747..2651436	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
HUW39_RS12690 (2651510)	2651510..2652457	+	948	WP_000854796.1	aldo/keto reductase	-
HUW39_RS12695 (2652560)	2652560..2652715	+	156	WP_001322415.1	hypothetical protein	-
HUW39_RS12700 (2652675)	2652675..2653094	+	420	WP_001322416.1	amidohydrolase family protein	-
HUW39_RS12705 (2653084)	2653084..2653236	+	153	WP_001322417.1	amidohydrolase family protein	-
HUW39_RS12710 (2653387)	2653387..2653959	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T162528 WP_001125415.1 NZ_CP056036:2647671-2648993 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T162528 NZ_CP071840:168135-169358 [Vibrio sp. SCSIO 43097]
GTGAGTAACAAAGAAAATAGTGTCGTCAGTTTTTACATTGACGACACTCCCACAGCTGAACAAGCTTCAAAAACCTTCCA
AGACGACTACGAACTGGACTTTCAAGATTTTATAGAAAGAACGGTAAATGAAAGTGCTGCTGAAGACTTCTCGTACTCAA
ATAATGGAGTAAAGAAAGCTGGAAGACGGCTTCGCAAGAGAGAGGGCGACCTCAAGTCAGCTACTGCTACAATCCAACAG
TTTCGAGCCGCACACGAAAAGCCATTAAACACTATCGCATATCTTGTTGGTCGCTGTTGCCGTGAGCTGGGTATAGATGT
TAAACCAGTTACACGGTTAAAGCGCTTAGAAACAATCGTAGATAAGTTGCAGCGTAAATCTCTTGATGGTGAAACGGCAA
ATGCTACTTGTGTAACCAATATGAATGATATTGGCGGCTGCAGGGCGATTTTTCCGGATTTAATTTCATTGGAGAAGGTG
AAATCTCAACTGCAACTTACAGTTGAGAAGGTAGGCCGAGTTAGAATTAAAGATATTGATGACTATATTACTAGTCCTAA
ACCTAATGATTGTGGTTACCGAAGCTTGCATGTGATTTATCGCTATGACCATGCTAGCGGTAAAAGTTTTAACATTGAGG
CTCAGCTACGTACTCGACTTCAACATCTTTGGGCTACTACAGTGGAAATTGTCGACATCCTAGAAGGTACCAAGATTAAG
ACTCACTCTCACAGTTCAAATGAAGGCAAGAGCAGTTTACAGGTTCAGTGGGAAGAATTGCTTTCGATAATGAGCCAATG
CATTGCTGATGCTGAAGGGGCTATAGAGTTAAGTGATATCGATAAAGCCGATTTTTCAAAGCGTTTGGTCGTCTTGGACC
GCGAAATCAATGCGCTAAATCGGTTGAGTAGTTTTAAAATTTTATCTGAAAAGGTTAATTCTTACTGTGATAGTTCCATT
GAGCACGTATTGCTAGTTATTAACGAAACTTCACGAGAATTGATCTTTAGTGAAGAGTTTGAAAATTACAGCCAAGCAAT
TTCAGTTTATAACGCAGCTGAGAAAGTCACTCGTAGCTACTCACGAGCAACCTCGAGACTCAATACCCTTCTCATATCCA
CAAAAAATATGGGCCAACTTTCGGAAGCCTACCCTAACTATCTAGGCGATTGTGCCTCATTCATCACTCTGCTTGAAGAC
ACAATGCTGTCTGGTGAAAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT162528 WP_001296726.1 NZ_CP056036:2647405-2647671 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT162528 NZ_CP071840:167671-168138 [Vibrio sp. SCSIO 43097]
ATGAAAGCATATCACACATACAGTGCTGTAGACGTTGCATGGAGCTTGCTCAAGCATGCGAAAGAGCAAGGTAAATGCTT
CTCCAATTTGCAGCTACAAAAACTTACCTATGTTTGTCATGGTTTATCATTGGCACACTTCCAGCGTCCTCTCGTCGTAG
ATGATGTATTTGCTTGGAAGTTTGGTCCAGTGGTGCCATCTGTTTACTTTCGCTTTAAATCTTACGGTAGTAGTGTGATC
ACTGAACAATGTGATGTAGCACTAGACCAAGAAAGTGAGTCTATTGTTAAAGATGTAGTCTCTAAGCTGGGCCATTTGAC
GGGGCCTCAACTTGTAGATTTAACTCACCGCGAAGGAAGTCCATGGCATCAGGTATGGGATGGCACACACCAAAAAGTGA
TTCCGGACCATTTGATTCAAGCCCATTACACTCAAATCAAACAATCCGGACGAACAGCTAGCCTGTGA

Structures

Toxin

• AlphaFold DB: U9YVT8