Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1014600..1015254 Replicon chromosome
Accession NZ_CP056036
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag HUW39_RS04885 Protein ID WP_000244769.1
Coordinates 1014847..1015254 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUW39_RS04880 Protein ID WP_000354046.1
Coordinates 1014600..1014866 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS04860 (1010688) 1010688..1012121 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
HUW39_RS04865 (1012166) 1012166..1012477 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HUW39_RS04870 (1012641) 1012641..1013300 + 660 WP_000250274.1 hemolysin III family protein -
HUW39_RS04875 (1013377) 1013377..1014357 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
HUW39_RS04880 (1014600) 1014600..1014866 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUW39_RS04885 (1014847) 1014847..1015254 + 408 WP_000244769.1 protein YgfX Toxin
HUW39_RS04890 (1015294) 1015294..1015815 - 522 WP_001055874.1 flavodoxin FldB -
HUW39_RS04895 (1015927) 1015927..1016823 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
HUW39_RS04900 (1016848) 1016848..1017558 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUW39_RS04905 (1017564) 1017564..1019297 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T162519 WP_000244769.1 NZ_CP056036:1014847-1015254 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T162519 NZ_CP071826:c2215052-2214633 [Burkholderia anthina]
ATGATTCTGGTCGATACCAACGTCATTGCCGAGCCGCTGCGACGCGAGCCGAGTGCGGCCGTGATCGCATGGCTCGACGC
GCAGAACGTCGAGACGCTGTTTCTTGCCGCGATCAGTCTGGCGGAGATGCGGTTCGGTGTGGCCGCGTTGCCGGAAGGGC
GCAGGCGAGATTGGCTGCAGCAAAGCATCGAGCAACGCGTGGTGCCGTTGTTTCGCGGCCGGATCCTGCCGTTCGATGAG
GCGGCCAGTCACGCGTATGCGAGCCTTCGTGCGCAGGCTCGCGCCATGGGCAGCGCGATGGCGTCTGCCGACGGTTTCAT
CGCCGCGACCGCCGCGGCGAACGGCCTGATCGTGGCTACGCGCGACGTCGCGCCGTTCGAAGCGGCGGGCCTGCGCGTGA
TCGATCCGTGGGCTGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT162519 WP_000354046.1 NZ_CP056036:1014600-1014866 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT162519 NZ_CP071826:c2215288-2215049 [Burkholderia anthina]
ATGCCCGTGATCACTGTTCGAAATCTGCCCGACGAGGTGCATCGCGCGCTTCGGATTCGTGCCGCGCAACATGGCCGCAG
CACTGAGGCCGAGGTTCGCGACATCCTCGAGCAAGCCGTTCGGCCCGGCGGGCGCCCCAGGCTGGGAACGCTGCTGGTTG
AAATCGGGAGGGAGGCCGGCGGCATCGATTTAGAGATTCAGCGCGGCACAGCGCCAACCGATCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References