Detailed information of TA system TA162497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 374218..374734
• Replicon: chromosome
• Accession: NZ_CP056030
• Organism: Pseudomonas eucalypticola strain NP-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HWQ56_RS01810
• Protein ID: WP_176569647.1
• Coordinates: 374447..374734 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HWQ56_RS01805
• Protein ID: WP_176569646.1
• Coordinates: 374218..374457 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ56_RS01770	369745..370332	+	588	WP_158156219.1	hypothetical protein	-
HWQ56_RS01775	370426..370869	-	444	WP_158156221.1	DUF3828 domain-containing protein	-
HWQ56_RS01780	370989..371408	-	420	WP_158156223.1	PH domain-containing protein	-
HWQ56_RS01785	371645..372223	+	579	WP_176569643.1	hypothetical protein	-
HWQ56_RS01790	372220..372618	+	399	WP_176569644.1	hypothetical protein	-
HWQ56_RS01795	372976..373284	-	309	WP_176569645.1	hypothetical protein	-
HWQ56_RS01800	373479..373796	+	318	WP_158156233.1	helix-turn-helix domain-containing protein	-
HWQ56_RS01805	374218..374457	+	240	WP_176569646.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HWQ56_RS01810	374447..374734	+	288	WP_176569647.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ56_RS01815	375223..375924	-	702	WP_176569648.1	HNH endonuclease	-
HWQ56_RS01820	375917..377026	-	1110	WP_176569649.1	AAA family ATPase	-
HWQ56_RS01825	377858..379378	+	1521	WP_176569650.1	SIR2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11237.15 Da
• Isoelectric Point: 10.4047

>T162497 WP_176569647.1 NZ_CP056030:374447-374734 [Pseudomonas eucalypticola]
MTYSLEFDARALKEWQKLGDTVRQQLKKKLATVLVNPRIEANRLHALRDCYKIKMRSSGYRLVYQVIDHEVVVFVVSVDK
RERELVYRKAAERLG

Nucleotide

• Length: 288 bp

>T162497 NZ_CP071819:4018815-4019033 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9044.39 Da
• Isoelectric Point: 4.4563

>AT162497 WP_176569646.1 NZ_CP056030:374218-374457 [Pseudomonas eucalypticola]
MASINIRIDDDLKARAYQELERLGVTPSELMRQALQYVAERGQLPFKPVLMTEDDEAMIATVRERLADPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT162497 NZ_CP071819:4018414-4018788 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG