Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 82864..83390 Replicon plasmid pRHBSTW-00975_2
Accession NZ_CP055884
Organism Enterobacter sp. RHBSTW-00975

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HV340_RS23665 Protein ID WP_000323025.1
Coordinates 83103..83390 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HV340_RS23660 Protein ID WP_000534858.1
Coordinates 82864..83103 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV340_RS23620 78005..78439 + 435 WP_033488162.1 conjugation system SOS inhibitor PsiB -
HV340_RS23625 78436..79164 + 729 WP_033488163.1 plasmid SOS inhibition protein A -
HV340_RS23630 79161..79487 + 327 WP_033488164.1 hypothetical protein -
HV340_RS23635 79544..79918 + 375 WP_181216570.1 hypothetical protein -
- 79978..80102 + 125 NuclAT_0 - -
- 79978..80102 + 125 NuclAT_0 - -
- 79978..80102 + 125 NuclAT_0 - -
- 79978..80102 + 125 NuclAT_0 - -
HV340_RS23640 80088..80153 + 66 WP_157844161.1 DUF5431 family protein -
HV340_RS23645 80222..81562 + 1341 WP_181216571.1 ISNCY family transposase -
HV340_RS23650 81999..82331 - 333 WP_001317460.1 FlxA-like family protein -
HV340_RS23655 82534..82839 - 306 WP_001326990.1 hypothetical protein -
HV340_RS23660 82864..83103 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HV340_RS23665 83103..83390 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HV340_RS23670 83462..83620 + 159 WP_008325141.1 type I toxin-antitoxin system Hok family toxin -
HV340_RS23675 84500..84793 + 294 WP_040119777.1 hypothetical protein -
HV340_RS23680 84810..85631 + 822 WP_015572081.1 DUF945 domain-containing protein -
HV340_RS23685 85660..86199 - 540 WP_045890596.1 lytic transglycosylase domain-containing protein -
HV340_RS23690 86798..87184 + 387 WP_023338043.1 hypothetical protein -
HV340_RS23695 87223..87864 - 642 WP_111969600.1 Arc family DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..137954 137954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T162495 WP_000323025.1 NZ_CP055884:83103-83390 [Enterobacter sp. RHBSTW-00975]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T162495 NZ_CP071819:1981297-1981399 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT162495 WP_000534858.1 NZ_CP055884:82864-83103 [Enterobacter sp. RHBSTW-00975]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT162495 NZ_CP071819:c1981406-1981261 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References