Detailed information of TA system TA162482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 63521..64122
• Replicon: plasmid pRHB28-C13_3
• Accession: NZ_CP055677
• Organism: Escherichia fergusonii strain RHB28-C13

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: HVY52_RS24570
• Protein ID: WP_001216045.1
• Coordinates: 63521..63901 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: HVY52_RS24575
• Protein ID: WP_001190712.1
• Coordinates: 63901..64122 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY52_RS24545	58962..60446	-	1485	WP_000124150.1	terminase	-
HVY52_RS24550	60446..61639	-	1194	WP_000219625.1	hypothetical protein	-
HVY52_RS24555	61725..62177	-	453	WP_001326849.1	late promoter-activating protein	-
HVY52_RS24560	62266..63309	-	1044	WP_112075408.1	DUF968 domain-containing protein	-
HVY52_RS24565	63337..63516	-	180	WP_001339207.1	hypothetical protein	-
HVY52_RS24570	63521..63901	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HVY52_RS24575	63901..64122	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HVY52_RS24580	64195..64584	-	390	WP_000506730.1	DNA repair protein	-
HVY52_RS24585	64759..65343	+	585	WP_023356284.1	hypothetical protein	-
HVY52_RS24590	65344..65706	+	363	WP_042102765.1	hypothetical protein	-
HVY52_RS24595	65718..65957	-	240	WP_023356286.1	DNA polymerase III subunit theta	-
HVY52_RS24600	65960..66160	-	201	WP_032145427.1	hypothetical protein	-
HVY52_RS24605	66272..67030	-	759	WP_032353390.1	hypothetical protein	-
HVY52_RS24610	67027..67401	-	375	WP_032353387.1	hypothetical protein	-
HVY52_RS24615	67408..67701	-	294	WP_121844072.1	hypothetical protein	-
HVY52_RS24620	67880..68113	-	234	WP_000517421.1	hypothetical protein	-
HVY52_RS24625	68196..68891	-	696	WP_052896298.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94009	94009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T162482 WP_001216045.1 NZ_CP055677:c63901-63521 [Escherichia fergusonii]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T162482 NZ_CP071805:c1805228-1804917 [Bifidobacterium pseudolongum]
ATGGAGGTAAGGACCACAAGCACATATGACACATGGCTCAACAAGCTCAAGGACAGACAGGCGAAGGCCAGGATCATCGC
GAAAACCGACATCATCCAAATGACCGGAACCATCACCGGCGACGTCGAACCCGTGGGCGGCGGCATCAGTGAACTGCGCT
TCCACTTTGGAGGCGGATACCGCATATACTTCGCCCACAAACACAACGAGATAATGTTGCTGATCGCAGGCGGCGACAAA
AGCAGCCAACCCAACGACATCAAACAAGCGCAACAGATACTCGACGCACTGAAAGGACAGCAACTATGGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT162482 WP_001190712.1 NZ_CP055677:c64122-63901 [Escherichia fergusonii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT162482 NZ_CP071805:c1804923-1804636 [Bifidobacterium pseudolongum]
ATGGTAACCATCAGCGATTGGGACGCCAGCAAATTCCTCAACAACGAGGACGACATCATCGCGTACCTCAACGCGGTCAT
GGAATACGACGACCCTGCCCTGTTCCAACAGGCCATCGGCAACGTCGCCAAGGCGCGCGGCATGGCACAGGTCGCCAAAG
AGGCGGGCGTGGGGCGCGAAAGCCTGTACAAGAGCCTATCGGCACAGGGGAACCCCTCTTTCCAAACCATCCTGAAAGTC
GTCAAAGCGCTGGGAGGGCGCATCAACATTGAACCCGCAACCGCATGA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6