Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3973780..3974356 Replicon chromosome
Accession NZ_CP055675
Organism Escherichia fergusonii strain RHB28-C13

Toxin (Protein)


Gene name shpA Uniprot ID A0A7W3EF74
Locus tag HVY52_RS19335 Protein ID WP_024256534.1
Coordinates 3974069..3974356 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HVY52_RS19330 Protein ID WP_000063156.1
Coordinates 3973780..3974082 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY52_RS19310 3969594..3970106 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
HVY52_RS19315 3970376..3972526 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HVY52_RS19320 3972576..3972779 + 204 WP_000467859.1 YbdD/YjiX family protein -
HVY52_RS19325 3972790..3973746 + 957 WP_001355122.1 GTPase -
HVY52_RS19330 3973780..3974082 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HVY52_RS19335 3974069..3974356 - 288 WP_024256534.1 BrnT family toxin Toxin
HVY52_RS19340 3974512..3975246 - 735 WP_000208601.1 Fic family protein -
HVY52_RS19345 3975509..3979021 + 3513 WP_181202857.1 type I restriction-modification system endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11241.74 Da        Isoelectric Point: 7.4020

>T162473 WP_024256534.1 NZ_CP055675:c3974356-3974069 [Escherichia fergusonii]
MPMEFEWDANKAKSNRMKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T162473 NZ_CP071802:c974824-974621 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATACGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT162473 WP_000063156.1 NZ_CP055675:c3974082-3973780 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT162473 NZ_CP071802:c975244-974876 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3EF74

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References