Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3827111..3827694 Replicon chromosome
Accession NZ_CP055599
Organism Escherichia fergusonii strain RHB41-C23

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag HV014_RS18530 Protein ID WP_000254750.1
Coordinates 3827359..3827694 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HV014_RS18525 Protein ID WP_000581937.1
Coordinates 3827111..3827359 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV014_RS18515 3823450..3824748 + 1299 WP_181198773.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HV014_RS18520 3824799..3827033 + 2235 WP_000226844.1 GTP diphosphokinase -
HV014_RS18525 3827111..3827359 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HV014_RS18530 3827359..3827694 + 336 WP_000254750.1 endoribonuclease MazF Toxin
HV014_RS18535 3827765..3828556 + 792 WP_001071671.1 nucleoside triphosphate pyrophosphohydrolase -
HV014_RS18540 3828784..3830421 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HV014_RS18545 3830509..3831807 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T162440 WP_000254750.1 NZ_CP055599:3827359-3827694 [Escherichia fergusonii]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T162440 NZ_CP071785:330534-330719 [Marinobacter sp. CA1]
ATGCAGCTGGCTGCGCTGGACACGGCCACTTCGGTGGAAGATATGGATATACCGGGTTTCCGGCTTTATCCATTGAAGGC
TAGAGACAAAGGGCGGTGGTCGATTCGTGTTACCGGAAATTGGCGCATGACGTTCGAATTTCAGGACGGTAACGCCTACA
TTCCTGATTATGAGGATTATCATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT162440 WP_000581937.1 NZ_CP055599:3827111-3827359 [Escherichia fergusonii]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT162440 NZ_CP071785:330719-331012 [Marinobacter sp. CA1]
ATGGCTATGCATAATCCGCCGCATCCTGGTGAATTCATTCGGGAAGTGTACCTGGAGCCTTTTGATATCAGTTCCCGTCA
GCTTGCTTCCAGTTTGGGGGTCTCTCCCTCCACTTTGTCTCGGCTGCTCAAAGGGAATAGTGGTATTAGCCCGGAAATGT
CGCTTCGACTATCCAAGGTTCTCGGGCGTTCTCCTGAGAGTTGGTTGGCGATGCAAGATATGTACGATCTATGGGTGGCC
CGCAACACGGTCAACCTGGATGGAATCCATCCCCTGGACTTCGAGGAAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References