Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2062491..2063067 Replicon chromosome
Accession NZ_CP055599
Organism Escherichia fergusonii strain RHB41-C23

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag HV014_RS10065 Protein ID WP_001350372.1
Coordinates 2062780..2063067 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HV014_RS10060 Protein ID WP_000063156.1
Coordinates 2062491..2062793 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV014_RS10040 2058305..2058817 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
HV014_RS10045 2059087..2061237 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HV014_RS10050 2061287..2061490 + 204 WP_000467859.1 YbdD/YjiX family protein -
HV014_RS10055 2061501..2062457 + 957 WP_001314406.1 GTPase -
HV014_RS10060 2062491..2062793 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HV014_RS10065 2062780..2063067 - 288 WP_001350372.1 BrnT family toxin Toxin
HV014_RS10070 2063264..2064430 + 1167 WP_000800829.1 restriction endonuclease -
HV014_RS10075 2064494..2066113 + 1620 WP_181199331.1 type I restriction-modification system subunit M -
HV014_RS10080 2066103..2067410 + 1308 WP_181199332.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T162429 WP_001350372.1 NZ_CP055599:c2063067-2062780 [Escherichia fergusonii]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T162429 NZ_CP071773:c3828053-3827700 [Proteus mirabilis]
ATGGCGAGTCTTGATGTATATATGAATGAATACTATATGGGCATCTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATACTGAAGATTGGGTTAATCAACCAGGAAGTCGTCCCATCTCTCTTTCGATGCCATTACAGCTTCAAGAATATCAAG
GAGACTGTGTCTATAATTTCTTTGATAATCTGTTACCTGATAATACTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCGAACTCAAATCAACCCTTTGACCTATTAGCGGCTATCGGTGCAGATACTGTTGGTGCATTAAGGTTACTTCCTAGTGG
CAGTACGCCTGCGGATATCAAAAAAATAGAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT162429 WP_000063156.1 NZ_CP055599:c2062793-2062491 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT162429 NZ_CP071773:c3828311-3828054 [Proteus mirabilis]
ATGATCATAATTAATGCCTATCAACTCAGTGTGCATCTAAAAGATGTCCGATTAACAAAAAAACTGTCTCAAAGTAAAGT
CGCCCAAAAAGTAGGGATCCGACAAGATACCGTATCCAATTTTGAACTTAATCCTAATTCCACCAAACTAGAAACCTTTT
TCAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References