Detailed information of TA system TA162413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 129353..129878
• Replicon: plasmid unnamed
• Accession: NZ_CP055510
• Organism: Klebsiella pneumoniae strain RHBSTW-00472

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9GHC5
• Locus tag: HV207_RS27650
• Protein ID: WP_009309918.1
• Coordinates: 129573..129878 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: HV207_RS27645
• Protein ID: WP_006788213.1
• Coordinates: 129353..129571 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV207_RS27625	125255..126532	-	1278	WP_009309915.1	HlyD family secretion protein	-
HV207_RS27630	127194..128072	+	879	WP_009309916.1	restriction endonuclease	-
HV207_RS27635	128113..128529	-	417	WP_009309917.1	type II toxin-antitoxin system VapC family toxin	-
HV207_RS27640	128526..128756	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV207_RS27645	129353..129571	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV207_RS27650	129573..129878	+	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV207_RS27655	129936..130247	+	312	WP_009309919.1	hypothetical protein	-
HV207_RS27660	130297..130632	+	336	WP_009309920.1	hypothetical protein	-
HV207_RS27665	130666..131682	+	1017	WP_009309921.1	hypothetical protein	-
HV207_RS27670	131880..132659	+	780	WP_023287113.1	site-specific integrase	-
HV207_RS27675	132717..132974	-	258	WP_009310077.1	hypothetical protein	-
HV207_RS27680	133103..133216	-	114	WP_014343462.1	hypothetical protein	-
HV207_RS27685	133319..134299	+	981	WP_014386491.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..333723	333723
	-	flank	IS/Tn	-	-	133319..134299	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11571.24 Da
• Isoelectric Point: 6.4661

>T162413 WP_009309918.1 NZ_CP055510:129573-129878 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T162413 NZ_CP071770:c2159289-2158870 [Acinetobacter towneri]
ATGCAAGTACAATATTTATTAGATACAAATATTTGTATTTACATTATTAAATACCGACCTGAAATTGTGCGTCAGCATTT
TGAAAAACATCTTCCGCATCGAAATATATTAATTTCTGTCATTACGTTGGGAGAATTACGTTTTGGTGCAGAAAAAAGTC
AACATAAAGAAAAGGCTTTAAAAGTCATAGATGAATTAACATCAATGATTCAAGTGGTTGAATTGGATGAAAATGTTGCA
CACCATTATGCAGAAATACGCCAGAACCTTGCATCTAAGGGGCAGATCATTGGCGCAAATGACTTATGGTTAGCAGCACA
TGCACGTGCAAATCATTGGGTCATGGTCACCAATAACGAAAAAGAATTTACTCGTGTCGATGGATTAAAAGTCGAAAACT
GGGTATCTAATCCTTTGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT162413 WP_006788213.1 NZ_CP055510:129353-129571 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT162413 NZ_CP071770:c2159519-2159289 [Acinetobacter towneri]
ATGACTACCGCAAAAGTTTTTATGACAGGACGAAGTCAAGCCATTCGATTGCCTAAAGAATTTCGCTTTGAAGGCAAAGA
AGTTGAAATTTTTCGTCGTGGTGATGAAGTCATTCTCCGTGAAAAACCCGTCACAGTAGATCGGTTATTTAAAGCGTTAT
CCCAAATGCCAAATGACTTTTATGCAGATGAAAGAGAAGATCTGCCGCCTCAAGAACGGGAAATGTTCTAA

Structures

Toxin

• AlphaFold DB: A0A7H9GHC5

Antitoxin

• AlphaFold DB: A0A1D8K3R4