Detailed information of TA system TA162412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 128113..128756
• Replicon: plasmid unnamed
• Accession: NZ_CP055510
• Organism: Klebsiella pneumoniae strain RHBSTW-00472

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV207_RS27635
• Protein ID: WP_009309917.1
• Coordinates: 128113..128529 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HV207_RS27640
• Protein ID: WP_001261276.1
• Coordinates: 128526..128756 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV207_RS27620	123157..125265	-	2109	WP_032427226.1	peptidase domain-containing ABC transporter	-
HV207_RS27625	125255..126532	-	1278	WP_009309915.1	HlyD family secretion protein	-
HV207_RS27630	127194..128072	+	879	WP_009309916.1	restriction endonuclease	-
HV207_RS27635	128113..128529	-	417	WP_009309917.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV207_RS27640	128526..128756	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV207_RS27645	129353..129571	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
HV207_RS27650	129573..129878	+	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	-
HV207_RS27655	129936..130247	+	312	WP_009309919.1	hypothetical protein	-
HV207_RS27660	130297..130632	+	336	WP_009309920.1	hypothetical protein	-
HV207_RS27665	130666..131682	+	1017	WP_009309921.1	hypothetical protein	-
HV207_RS27670	131880..132659	+	780	WP_023287113.1	site-specific integrase	-
HV207_RS27675	132717..132974	-	258	WP_009310077.1	hypothetical protein	-
HV207_RS27680	133103..133216	-	114	WP_014343462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..333723	333723
	-	flank	IS/Tn	-	-	133319..134299	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T162412 WP_009309917.1 NZ_CP055510:c128529-128113 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKGSPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T162412 NZ_CP071770:1950892-1951200 [Acinetobacter towneri]
TTGATTAAGAGCTTTAAACATAAAGGTCTTCAGGCTTTTTTCCAAACTGGTTCAACAGCAGGTATTCAAGCAGCGCATTC
TACAAAATTACGTTTAATACTTGCAGCACTACATGCTGCATCTACGGTGAATGATTTGCGAACTCCACCCAATTGGCGTT
TACATAAATTGAATGGAAATCTACAAGATCAATGGTCACTAACAGTAAATGGTAATTGGCGTGTAATTTTTAAATTTGAA
GATGGTGATGTCTACATTGTAGATTATTTGGATTATCACATAGCTTCTAGCGCAGGAGTACAAAGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT162412 WP_001261276.1 NZ_CP055510:c128756-128526 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT162412 NZ_CP071770:1951197-1951478 [Acinetobacter towneri]
ATGAATATGATGTTTAATGCACCTCATCCGGGCGAAATGTTGCGCGAATATATTGGTGAAACTTCTGTGACTGAAGTGGC
TCAAGCACTTGGAGTGACACGTGCAAACCTATCTCGCATTTTGAATGGTCATACTGGAATTTCTGCAGATATGGCGATTC
GTTTGAGTGAAGCCTTAGAAACCTCACCAGAGTTTTGGTTGAATTTACAAATGCAGCATGATTTATGGCTTGCCAGTCAA
CGCCAGCGACCTATGATCAAAAAATTGGTGGCAAGTGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023