Detailed information of TA system TA162410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 84539..85275
• Replicon: plasmid unnamed
• Accession: NZ_CP055510
• Organism: Klebsiella pneumoniae strain RHBSTW-00472

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV207_RS27390
• Protein ID: WP_181247937.1
• Coordinates: 84539..85021 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HV207_RS27395
• Protein ID: WP_003026799.1
• Coordinates: 85009..85275 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV207_RS27375	80671..81303	+	633	WP_001567369.1	hypothetical protein	-
HV207_RS27380	81332..82735	-	1404	WP_181247936.1	ISNCY-like element ISKpn21 family transposase	-
HV207_RS27385	82847..84379	+	1533	WP_009310015.1	IS3-like element ISKpn38 family transposase	-
HV207_RS27390	84539..85021	-	483	WP_181247937.1	GNAT family N-acetyltransferase	Toxin
HV207_RS27395	85009..85275	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HV207_RS27400	85451..85705	-	255	WP_004118702.1	hypothetical protein	-
HV207_RS27405	85781..86038	-	258	WP_004098928.1	hypothetical protein	-
HV207_RS27410	86087..86290	-	204	WP_004098931.1	hemolysin expression modulator Hha	-
HV207_RS27415	86321..86689	-	369	WP_009310054.1	hypothetical protein	-
HV207_RS27420	86733..87227	-	495	WP_009310053.1	hypothetical protein	-
HV207_RS27425	87258..87824	-	567	WP_009310052.1	hypothetical protein	-
HV207_RS27430	87821..88084	-	264	WP_009310051.1	hypothetical protein	-
HV207_RS27435	88374..89912	-	1539	WP_004196907.1	IS66-like element ISEc22 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..333723	333723
	-	inside	IScluster/Tn	-	-	69296..90709	21413

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17311.89 Da
• Isoelectric Point: 9.5822

>T162410 WP_181247937.1 NZ_CP055510:c85021-84539 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGGGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T162410 NZ_CP071770:1371467-1371802 [Acinetobacter towneri]
ATGAATCAAGGCGATATTTATTTGATTTCATTAGATCCAACACTTGGACATGAACAACAAGGTAAGCGACCTGTATTTGT
TATTTCACCTAAGTCATTTAATCTTATTACTAAAATGCCGATTGTTTTGCCTATTACTTCTGGTGGAAACTTTGCACGAA
ATATGGGTTTCGCTGTAGAGCTTATTGGTGGGAAAACTACAGGTATTGTGCGTTGTGATCAATCTAGAGCTTTAGATTTT
GAAGCTAGACACGCGCGGTTTTTAGAATCCTCGCACGCAGATGTTTTGGATGAGGTTTTAGCAAGATATATCGCAATTTT
TTCACCTGAAGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT162410 WP_003026799.1 NZ_CP055510:c85275-85009 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT162410 NZ_CP071770:1371219-1371467 [Acinetobacter towneri]
ATGATTCATACAAACTTGAGAAAAGTAGGTGGTTCGGTCATGCTTGCCGTACCGCCTACATTATTAAAAAGTTTAGGTTT
AGATGCGGGAAAGGAAGTTTCTCTGAGCATTGTGAATGGTAAGTTAGTGGTCGAACCTAAAGCGAAGCCATCTTATAAAT
TGGCTGACTTGCTGCAAGAGCATCAAGCTTTAGCATTAGAGCAAGATGCGTGGCATGATGTAAAAGCAGTTGGGCGAGAA
GAAATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66