Detailed information of TA system TA162391

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 652727..653430
• Replicon: chromosome
• Accession: NZ_CP055509
• Organism: Klebsiella pneumoniae strain RHBSTW-00472

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV207_RS03210
• Protein ID: WP_009309675.1
• Coordinates: 653089..653430 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV207_RS03205
• Protein ID: WP_009309674.1
• Coordinates: 652727..653068 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV207_RS03180	648243..649405	+	1163	WP_087728260.1	IS3 family transposase	-
HV207_RS03185	649806..650669	+	864	WP_009309669.1	hypothetical protein	-
HV207_RS03190	650867..651688	+	822	WP_009309670.1	DUF945 domain-containing protein	-
HV207_RS03195	651719..652161	+	443	Protein_626	antirestriction protein	-
HV207_RS03200	652174..652716	+	543	WP_009309673.1	DNA repair protein RadC	-
HV207_RS03205	652727..653068	+	342	WP_009309674.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV207_RS03210	653089..653430	+	342	WP_009309675.1	TA system toxin CbtA family protein	Toxin
HV207_RS03215	653723..654760	-	1038	WP_181247888.1	tyrosine-type recombinase/integrase	-
HV207_RS03220	654767..655351	-	585	WP_057222572.1	phage repressor protein CI	-
HV207_RS03225	655471..655692	+	222	WP_004195891.1	hypothetical protein	-
HV207_RS03230	655723..656232	+	510	WP_181247889.1	phage regulatory CII family protein	-
HV207_RS03235	656240..656440	+	201	WP_181247890.1	DUF2724 domain-containing protein	-
HV207_RS03240	656521..656808	+	288	WP_181247931.1	hypothetical protein	-
HV207_RS03245	656874..657098	+	225	WP_143911932.1	DUF2732 family protein	-
HV207_RS03250	657098..657325	+	228	WP_023317535.1	TraR/DksA family transcriptional regulator	-
HV207_RS03255	657322..657903	+	582	WP_181247891.1	3'-5' exonuclease	-
HV207_RS03260	657900..658172	+	273	WP_135712807.1	DUF5405 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	645016..730763	85747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12822.76 Da
• Isoelectric Point: 9.6542

>T162391 WP_009309675.1 NZ_CP055509:653089-653430 [Klebsiella pneumoniae]
MKTLPATISRAAKPCLSPVAAWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAEITLADAVNFLVDKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLRRSRNNAVS

Nucleotide

• Length: 342 bp

>T162391 NZ_CP071767:93665-93961 [Acinetobacter towneri]
ATGATTGAAATTAAACGTCTTCCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGTATCCGCCTGAA
TCGTCGATTAGATAAGGTTCAACGTGGAAACTGGGGAGACATTAAACCTCTCCAAGATGGCGTTTGGGAAATGAGAGAAT
TCTTCGGTTCTGGATGGAGGATGTATTACATCCAACATGGTGACGTTGTGATTGTAATGCTTGGTGGTGGAGATAAATCA
ACTCAACAACAAGACATCGATCGGGCAGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12735.41 Da
• Isoelectric Point: 6.3157

>AT162391 WP_009309674.1 NZ_CP055509:652727-653068 [Klebsiella pneumoniae]
MKSTESENDLFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGAFSNAESLKLDEVFPRFISQMESMLTIGEMNPHH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT162391 NZ_CP071767:93963-94253 [Acinetobacter towneri]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAAAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGGCGTGAAGCACTCTACAAAGCCCTACGTCAGGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAACTCACAGTACAACACGCTTAA