Detailed information of TA system TA162387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 200405..200931
• Replicon: plasmid pRHBSTW-00574_2
• Accession: NZ_CP055452
• Organism: Klebsiella pneumoniae strain RHBSTW-00574

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HV239_RS26875
• Protein ID: WP_000323025.1
• Coordinates: 200405..200692 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W3H0
• Locus tag: HV239_RS26880
• Protein ID: WP_004196370.1
• Coordinates: 200692..200931 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV239_RS26845	196076..196393	-	318	WP_015065519.1	hypothetical protein	-
HV239_RS26850	196408..196758	-	351	WP_032425551.1	hypothetical protein	-
HV239_RS26855	196755..197027	-	273	WP_032425552.1	hypothetical protein	-
HV239_RS26860	197883..198995	-	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
HV239_RS26865	199074..199232	-	159	WP_014343509.1	type I toxin-antitoxin system Hok family toxin	-
HV239_RS26870	199304..200338	+	1035	Protein_226	IS481-like element ISKpn28 family transposase	-
HV239_RS26875	200405..200692	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HV239_RS26880	200692..200931	-	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HV239_RS26885	201182..201547	+	366	WP_009651956.1	hypothetical protein	-
HV239_RS26890	201591..202328	+	738	WP_008460272.1	HupE/UreJ family protein	-
HV239_RS26895	202342..203031	+	690	WP_004196322.1	hypothetical protein	-
HV239_RS26900	203062..204438	-	1377	Protein_232	chromate efflux transporter	-
HV239_RS26905	204395..205372	-	978	WP_004196334.1	chromate resistance protein	-
HV239_RS26910	205402..205594	+	193	Protein_234	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF	1..244871	244871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T162387 WP_000323025.1 NZ_CP055452:c200692-200405 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T162387 NZ_CP071766:c1323792-1323484 [Acinetobacter towneri]
TTGATTAAGAGCTTTAAACATAAAGGTCTTCAGGCTTTTTTCCAAACTGGTTCAACAGCAGGTATTCAAGCAGCGCATTC
TACAAAATTACGTTTAATACTTGCAGCACTACATGCTGCATCTACGGTGAATGATTTGCGAACTCCACCCAATTGGCGTT
TACATAAATTGAATGGAAATCTACAAGATCAATGGTCACTAACAGTAAATGGTAATTGGCGTGTAATTTTTAAATTTGAA
GATGGTGATGTCTACATTGTAGATTATTTGGATTATCACATAGCTTCTAGCGCAGGAGTACAAAGATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9018.43 Da
• Isoelectric Point: 4.3894

>AT162387 WP_004196370.1 NZ_CP055452:c200931-200692 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT162387 NZ_CP071766:c1323487-1323206 [Acinetobacter towneri]
ATGAATATGATGTTTAATGCACCTCATCCGGGCGAAATGTTGCGCGAATATATTGGTGAAACTTCTGTGACTGAAGTGGC
TCAAGCACTTGGAGTGACACGTGCAAACCTATCTCGCATTTTGAATGGTCATACTGGAATTTCTGCAGATATGGCGATTC
GTTTGAGTGAAGCCTTAGAAACCTCACCAGAGTTTTGGTTGAATTTACAAATGCAGCATGATTTATGGCTTGCCAGTCAA
CGCCAGCGACCTATGATCAAAAAATTGGTGGCAAGTGTTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A2J4QRH4