Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 110833..111476 Replicon plasmid pRHBSTW-00574_2
Accession NZ_CP055452
Organism Klebsiella pneumoniae strain RHBSTW-00574

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HV239_RS26375 Protein ID WP_001044770.1
Coordinates 110833..111249 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HV239_RS26380 Protein ID WP_001261282.1
Coordinates 111246..111476 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV239_RS26355 106202..106552 + 351 WP_004187110.1 DUF305 domain-containing protein -
HV239_RS26360 106738..107646 - 909 WP_032425603.1 HNH endonuclease -
HV239_RS26365 108190..109212 - 1023 WP_000361404.1 helicase UvrD -
HV239_RS26370 109197..110759 - 1563 WP_080892739.1 AAA family ATPase -
HV239_RS26375 110833..111249 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HV239_RS26380 111246..111476 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV239_RS26385 111433..111663 + 231 Protein_129 hypothetical protein -
HV239_RS26390 111734..112714 + 981 WP_009310076.1 IS5-like element ISKpn26 family transposase -
HV239_RS26395 112863..113924 - 1062 Protein_131 HlyD family secretion protein -
HV239_RS26400 114586..115464 + 879 WP_009309916.1 restriction endonuclease -
HV239_RS26405 115505..115921 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
HV239_RS26410 115918..116148 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF 1..244871 244871
- flank IS/Tn - - 111734..112714 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T162385 WP_001044770.1 NZ_CP055452:c111249-110833 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T162385 NZ_CP071766:c215403-215155 [Acinetobacter towneri]
ATGGGTAAAACAGAAAAACTGTTGGATAAAATTGGGAATTCAAAAAACACATTTCCGTATAAGGACTTAGTTGTTTTACT
AAGTCAGCTCGGCTATGAAAAGTTTGAAATGGCAGGCTCTCGCGTCCGTTTTTATAACCTTAAAACCGAGCACATGATTT
TACTGCATCGACCTCACCCTGAAAATGAAATCAAAGGTGGGGCATTGAAAGCTGTAAAGCAAGCACTCAAGCAGGAGGGT
TTTTTATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT162385 WP_001261282.1 NZ_CP055452:c111476-111246 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT162385 NZ_CP071766:c215158-214823 [Acinetobacter towneri]
ATGAGCTATTTTAAATACAAAGGTTATCTGGGGACGATTGAACCAGACCTTGAAACAGGTGAGTTGTTTGGAAAATTGGC
GTTCATTCGAGATCTTATTACTTATGAGGCTGAAACCTTAAAAACACTTGAGCAAGCTTTTCAGGAATCGGTAGATGGAT
ATTTAGATTCTTGTGCAGAATTAGGGAAGAAACCTGATCAGCCATTCAAGGGAACATTTAATGTTCGTATCAGTCCTGAA
TTACATCGCAAAGCTGTTTTAGCTTCGGAGGAGAGTTCTTTGAATGCATTTGTGAGTGATGCAATTCAAGAGAAATTAAT
GCGCTTAGGTGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References