Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 75465..76201 Replicon plasmid pRHBSTW-00574_2
Accession NZ_CP055452
Organism Klebsiella pneumoniae strain RHBSTW-00574

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag HV239_RS26195 Protein ID WP_004098919.1
Coordinates 75719..76201 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A455TK27
Locus tag HV239_RS26190 Protein ID WP_020804426.1
Coordinates 75465..75731 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV239_RS26170 70903..72318 - 1416 WP_023329023.1 AAA family ATPase -
HV239_RS26175 72315..73907 - 1593 Protein_87 ATP-binding protein -
HV239_RS26180 73976..74956 + 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
HV239_RS26185 75080..75289 + 210 WP_050482669.1 hypothetical protein -
HV239_RS26190 75465..75731 + 267 WP_020804426.1 DUF1778 domain-containing protein Antitoxin
HV239_RS26195 75719..76201 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
HV239_RS26200 76402..77805 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV239_RS26205 77834..78466 - 633 WP_001567369.1 hypothetical protein -
HV239_RS26210 78687..79430 + 744 Protein_94 IS3-like element ISKpn38 family transposase -
HV239_RS26215 79456..80424 - 969 WP_000654814.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF 1..244871 244871
- inside IScluster/Tn - - 64948..81678 16730


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T162384 WP_004098919.1 NZ_CP055452:75719-76201 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T162384 NZ_CP071765:2900-3187 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10124.47 Da        Isoelectric Point: 4.3108

>AT162384 WP_020804426.1 NZ_CP055452:75465-75731 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV

Download         Length: 267 bp

>AT162384 NZ_CP071765:3174-3488 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A455TK27

References