Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 22269..22794 Replicon plasmid pRHBSTW-00574_2
Accession NZ_CP055452
Organism Klebsiella pneumoniae strain RHBSTW-00574

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag HV239_RS25865 Protein ID WP_004197633.1
Coordinates 22269..22574 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV239_RS25870 Protein ID WP_006788213.1
Coordinates 22576..22794 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV239_RS25825 17614..17700 - 87 Protein_17 EAL domain-containing protein -
HV239_RS25830 17848..18828 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
HV239_RS25835 18931..19044 + 114 WP_014343462.1 hypothetical protein -
HV239_RS25840 19173..19430 + 258 WP_009310077.1 hypothetical protein -
HV239_RS25845 19488..20267 - 780 WP_023287113.1 site-specific integrase -
HV239_RS25850 20465..21481 - 1017 WP_009309921.1 hypothetical protein -
HV239_RS25855 21515..21850 - 336 WP_009309920.1 hypothetical protein -
HV239_RS25860 21900..22211 - 312 WP_023287114.1 hypothetical protein -
HV239_RS25865 22269..22574 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
HV239_RS25870 22576..22794 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV239_RS25875 23365..25419 - 2055 WP_181469659.1 hypothetical protein -
HV239_RS25880 26069..27049 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
HV239_RS25885 27199..27684 - 486 Protein_29 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF 1..244871 244871
- flank IS/Tn - - 17848..18828 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T162383 WP_004197633.1 NZ_CP055452:c22574-22269 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T162383 NZ_CP071763:3957013-3957300 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT162383 WP_006788213.1 NZ_CP055452:c22794-22576 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT162383 NZ_CP071763:3957287-3957601 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References