Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5181803..5182428 Replicon chromosome
Accession NZ_CP055451
Organism Klebsiella pneumoniae strain RHBSTW-00574

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV239_RS25375 Protein ID WP_002882817.1
Coordinates 5181803..5182186 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV239_RS25380 Protein ID WP_004150355.1
Coordinates 5182186..5182428 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV239_RS25360 5179169..5180071 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV239_RS25365 5180068..5180703 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV239_RS25370 5180700..5181629 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV239_RS25375 5181803..5182186 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV239_RS25380 5182186..5182428 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV239_RS25385 5182633..5183550 + 918 WP_060415997.1 alpha/beta hydrolase -
HV239_RS25390 5183564..5184505 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV239_RS25395 5184550..5184987 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV239_RS25400 5184984..5185844 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV239_RS25405 5185838..5186437 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T162382 WP_002882817.1 NZ_CP055451:c5182186-5181803 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T162382 NZ_CP071763:1216147-1216329 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT162382 WP_004150355.1 NZ_CP055451:c5182428-5182186 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT162382 NZ_CP071763:1216422-1216826 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References