Detailed information of TA system TA162379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4592034..4592844
• Replicon: chromosome
• Accession: NZ_CP055451
• Organism: Klebsiella pneumoniae strain RHBSTW-00574

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV239_RS22570
• Protein ID: WP_060416080.1
• Coordinates: 4592034..4592567 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HV239_RS22575
• Protein ID: WP_002887278.1
• Coordinates: 4592578..4592844 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV239_RS22565	4590865..4591986	+	1122	WP_060416081.1	cupin domain-containing protein	-
HV239_RS22570	4592034..4592567	-	534	WP_060416080.1	GNAT family N-acetyltransferase	Toxin
HV239_RS22575	4592578..4592844	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HV239_RS22580	4592947..4594380	-	1434	WP_060416079.1	Cu(+)/Ag(+) sensor histidine kinase	-
HV239_RS22585	4594370..4595053	-	684	WP_004214143.1	copper response regulator transcription factor CusR	-
HV239_RS22590	4595225..4596610	+	1386	WP_060416078.1	efflux transporter outer membrane subunit	-
HV239_RS22595	4596628..4596972	+	345	WP_025987660.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19699.56 Da
• Isoelectric Point: 5.2466

>T162379 WP_060416080.1 NZ_CP055451:c4592567-4592034 [Klebsiella pneumoniae]
MEQQLTIEMIAGAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLCLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T162379 NZ_CP071762:2518891-2519112 [Burkholderia pseudomallei]
ATGGAGTGTCGTTGGCGCTTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAG
TGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGC
ACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT162379 WP_002887278.1 NZ_CP055451:c4592844-4592578 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT162379 NZ_CP071762:2519096-2519380 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1