Detailed information of TA system TA162363

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4342974..4343653
• Replicon: chromosome
• Accession: NZ_CP055418
• Organism: Citrobacter sp. RHBSTW-00944

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV334_RS21035
• Protein ID: WP_181220861.1
• Coordinates: 4342974..4343315 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: HV334_RS21040
• Protein ID: WP_006812013.1
• Coordinates: 4343336..4343653 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV334_RS21015	4338305..4340050	+	1746	WP_019077713.1	DNA primase	-
HV334_RS21020	4340215..4342059	+	1845	WP_016154549.1	RNA polymerase sigma factor RpoD	-
HV334_RS21025	4342105..4342611	-	507	WP_103767095.1	G/U mismatch-specific DNA glycosylase	-
HV334_RS21035	4342974..4343315	-	342	WP_181220861.1	TA system toxin CbtA family protein	Toxin
HV334_RS21040	4343336..4343653	-	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV334_RS21045	4343673..4343894	-	222	WP_001548171.1	DUF987 domain-containing protein	-
HV334_RS21050	4343903..4344379	-	477	WP_000811693.1	RadC family protein	-
HV334_RS21055	4344395..4344853	-	459	WP_001548150.1	antirestriction protein	-
HV334_RS21060	4344951..4345190	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV334_RS21065	4345267..4345734	-	468	WP_001547765.1	hypothetical protein	-
HV334_RS21070	4345757..4346200	-	444	WP_181220862.1	hypothetical protein	-
HV334_RS21075	4346200..4346436	-	237	WP_001144031.1	hypothetical protein	-
HV334_RS21080	4346477..4347178	-	702	WP_181220863.1	WYL domain-containing protein	-
HV334_RS21085	4347395..4348084	-	690	Protein_4124	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp/tssD	4342105..4378641	36536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12979.03 Da
• Isoelectric Point: 10.4476

>T162363 WP_181220861.1 NZ_CP055418:c4343315-4342974 [Citrobacter sp. RHBSTW-00944]
MKTLPATTQRAAKPCLASVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T162363 NZ_CP071755:c3053675-3053406 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT162363 WP_006812013.1 NZ_CP055418:c4343653-4343336 [Citrobacter sp. RHBSTW-00944]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT162363 NZ_CP071755:c3053422-3053138 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: R8WMM2