Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3766564..3767184 Replicon chromosome
Accession NZ_CP055418
Organism Citrobacter sp. RHBSTW-00944

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV334_RS18120 Protein ID WP_002892050.1
Coordinates 3766966..3767184 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7L6U5G9
Locus tag HV334_RS18115 Protein ID WP_019076175.1
Coordinates 3766564..3766938 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV334_RS18105 3761711..3762904 + 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV334_RS18110 3762927..3766076 + 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV334_RS18115 3766564..3766938 + 375 WP_019076175.1 Hha toxicity modulator TomB Antitoxin
HV334_RS18120 3766966..3767184 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV334_RS18125 3767365..3767916 + 552 WP_181220739.1 maltose O-acetyltransferase -
HV334_RS18130 3768033..3768503 + 471 WP_047416865.1 YlaC family protein -
HV334_RS18135 3768582..3768722 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV334_RS18140 3768724..3768984 - 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV334_RS18145 3769173..3770726 + 1554 WP_047501906.1 EAL domain-containing protein -
HV334_RS18150 3770778..3771131 - 354 WP_003831033.1 DUF1428 family protein -
HV334_RS18155 3771196..3771825 - 630 WP_016155872.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162360 WP_002892050.1 NZ_CP055418:3766966-3767184 [Citrobacter sp. RHBSTW-00944]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T162360 NZ_CP071754:c1831765-1831514 [Burkholderia pseudomallei]
ATGCAGCGACTGCGCGATTTTCTGCGACCGAAGAATCCTGACGCCGCAAGGCGAGCTGGCGAAGCGATTAGACAAGGCGT
ACAGGCTCTCGGTACTCACCCTCGAATGGGGCGTCTCGTAGAAGATCTACCCGAGCAGTATCGGGAGTGGCCCATCGATT
TCGGGGACAGTGGTTACGTGGCCCGGTATCGCTTCGATGGCGACGCCGTCACGATCTTGGCTGTCCGTCATCAGAAGGAA
GCGGGGTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14413.20 Da        Isoelectric Point: 5.5653

>AT162360 WP_019076175.1 NZ_CP055418:3766564-3766938 [Citrobacter sp. RHBSTW-00944]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT162360 NZ_CP071754:c1832070-1831792 [Burkholderia pseudomallei]
ATGGTTACGGCAACATCCATCAAGCTTGATGATGAACTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCTCGTCGCCGCAC
CCCGCATTGGATCATGCGTGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGTATTGAACCAGGATACGC
TCAAGGCATGGGATGAATTTCAGGAGACTGGCCTGCACGCAACCGCCGAGGAGGTGGATAAGTGGCTTGCGAGCTGGGGA
ACTGAAAACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U5G9

References