Detailed information of TA system TA162351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2539528..2540164
• Replicon: chromosome
• Accession: NZ_CP055418
• Organism: Citrobacter sp. RHBSTW-00944

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV334_RS12205
• Protein ID: WP_049259794.1
• Coordinates: 2539528..2539716 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV334_RS12210
• Protein ID: WP_131341011.1
• Coordinates: 2539748..2540164 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV334_RS12185	2536306..2536536	-	231	WP_016153158.1	DUF2554 family protein	-
HV334_RS12190	2536726..2536851	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV334_RS12195	2536851..2537861	-	1011	WP_141875205.1	cytochrome d ubiquinol oxidase subunit II	-
HV334_RS12200	2537861..2539264	-	1404	WP_047417372.1	cytochrome ubiquinol oxidase subunit I	-
HV334_RS12205	2539528..2539716	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV334_RS12210	2539748..2540164	+	417	WP_131341011.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV334_RS12215	2540257..2541666	+	1410	WP_019076828.1	PLP-dependent aminotransferase family protein	-
HV334_RS12220	2541996..2543141	+	1146	WP_181221815.1	ABC transporter substrate-binding protein	-
HV334_RS12225	2543158..2544174	+	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
HV334_RS12230	2544175..2545119	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T162351 WP_049259794.1 NZ_CP055418:2539528-2539716 [Citrobacter sp. RHBSTW-00944]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T162351 NZ_CP071750:2407950-2408306 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15034.31 Da
• Isoelectric Point: 4.7119

>AT162351 WP_131341011.1 NZ_CP055418:2539748-2540164 [Citrobacter sp. RHBSTW-00944]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGAEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT162351 NZ_CP071750:2407651-2407947 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGAAAAAATTATCGTCGTGGTGTTGTGCGGTGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9