Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 212411..213054 Replicon plasmid unnamed
Accession NZ_CP055327
Organism Klebsiella michiganensis strain RHBSTW-00409

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag HV187_RS31235 Protein ID WP_021312768.1
Coordinates 212638..213054 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag HV187_RS31230 Protein ID WP_021312767.1
Coordinates 212411..212641 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV187_RS31210 208672..209427 - 756 WP_001568031.1 replication initiation protein RepE -
HV187_RS31215 209962..210744 - 783 WP_020277927.1 site-specific integrase -
HV187_RS31220 210756..211481 - 726 WP_065813277.1 hypothetical protein -
HV187_RS31225 211497..211796 - 300 WP_052433691.1 hypothetical protein -
HV187_RS31230 212411..212641 + 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV187_RS31235 212638..213054 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
HV187_RS31240 213152..213385 - 234 WP_089617563.1 helix-turn-helix domain-containing protein -
HV187_RS31245 213391..214095 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HV187_RS31250 214536..214856 + 321 WP_001137910.1 DUF134 domain-containing protein -
HV187_RS31255 214831..215292 + 462 WP_000626969.1 hypothetical protein -
HV187_RS31260 215453..215890 + 438 WP_016241611.1 PaaI family thioesterase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..215915 215915
- flank IS/Tn - - 213391..214095 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T162347 WP_021312768.1 NZ_CP055327:212638-213054 [Klebsiella michiganensis]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T162347 NZ_CP071749:1498553-1498966 [Burkholderia pseudomallei]
ATGAGCGGGGCTGACAAGACGAAAGTATTCGTGGACAGCAACGTCGTGCTGTATCTGCTTTCCGGTGATACCGCTAAAGC
GGATCGTGCGGAGGCTCTGTTGCGGCGCCGGCCGACGATCAGCGTTCAGGTGCTGAACGAAGTCACGAACGTCTGCGTGC
GTAAGCTCGGGATGGCGTGGCGTGAGGTCGAACAGTTCCTGGAACTTGTGCGGCAGTTTTGCGTGGTCGCGCCGCTTACA
ATCGAAATGCATGATTTGGCGCGGCGCATTGCGGAACGCTATGGTCTGGCATTCTATGATGCGTGCATCGTAGCAGCCGC
GTCGATCGAAGGATGCCACGCCCTCTACACGGAAGACATGCATCACGGATTGATCATCGAAGAAAGCTTGACGCTGCGTA
ACCCTTTCGCGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT162347 WP_021312767.1 NZ_CP055327:212411-212641 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT162347 NZ_CP071749:1498333-1498566 [Burkholderia pseudomallei]
ATGCAGGTCGCTAAATGGGGCAATAGCCTTGCCGTCCGCCTTCCGTCGAGCCTGGTCGAAGCGCTTGAGCTGCGCGAGGG
AGACGATATCGAGATTGTCATCGACGCGCCGCGCACCTTTGCGGTACGCAAGAAGCCGGGGGCGGATGAACTGCTGGAGC
GGTTGCGCCGCTTCCGCGGCAAGCTGCCGGCCGATTTCAAGTTCAGCCGGGACGAGGCGAATGAGCGGGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References