Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 12566..13302 Replicon plasmid unnamed
Accession NZ_CP055327
Organism Klebsiella michiganensis strain RHBSTW-00409

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV187_RS30190 Protein ID WP_003026803.1
Coordinates 12566..13048 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV187_RS30195 Protein ID WP_003026799.1
Coordinates 13036..13302 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV187_RS30170 8018..8650 + 633 WP_001567369.1 hypothetical protein -
HV187_RS30175 8679..10082 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV187_RS30180 10301..10933 + 633 WP_001567369.1 hypothetical protein -
HV187_RS30185 10962..12365 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV187_RS30190 12566..13048 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV187_RS30195 13036..13302 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV187_RS30200 13478..13732 - 255 WP_004152108.1 hypothetical protein -
HV187_RS30205 13808..14065 - 258 WP_004152107.1 hypothetical protein -
HV187_RS30210 14114..14317 - 204 WP_004152106.1 hemolysin expression modulator Hha -
HV187_RS30215 14351..14718 - 368 Protein_18 hypothetical protein -
HV187_RS30220 14762..15256 - 495 WP_016831217.1 DNA-binding protein -
HV187_RS30225 15287..15862 - 576 WP_020317495.1 hypothetical protein -
HV187_RS30230 15850..16119 - 270 WP_004152102.1 hypothetical protein -
HV187_RS30235 16477..16827 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HV187_RS30240 16877..17239 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..215915 215915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T162346 WP_003026803.1 NZ_CP055327:c13048-12566 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T162346 NZ_CP071749:193830-194174 [Burkholderia pseudomallei]
ATGAAACAAAATTGGACAGTCGTCTATTACAACGAGCGCGTCAAACGCGACGTCTTCGCGCTGCCGGCAGGGATTCTGGC
GGACTATCTGCGGCTCCTGGACTTGATGCAGGAGTTCGGCGCGGATTTGCGTATGCCGCACTCGCGAGCAATGGGGGACG
GATTGTTTGAGTTGCGCCCGAAGGGGCGAGAGGGCATCGGGCGCGTGCTTTATTGCACGCACTTTGGGCGGCACGTCGTG
GTGCTGCATTCGTTTGTGAAGAAGACGCAGGAGGCGCCGCAGAAAGAACTGCGCACCGCCCGAGCGCGTTTGAGCGAGGT
ACGAAATGGTGAAAGCGGTTGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT162346 WP_003026799.1 NZ_CP055327:c13302-13036 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT162346 NZ_CP071749:194212-194487 [Burkholderia pseudomallei]
ATGACGGATGACACTGAGCGTCTACTGGCAAAGCCTGGTGTCAAAGCACAATACGATGCGCTAGAAGATGAATACAGCGC
GCTTCGAGCCATCTTGACGGCTCGGCAAGACGCCGGCTTGACGCAGGCTCAGGTGGCCGAGCGCATGGGAACGACAGCTT
CTGCAGTTTCGCGATTGGAAGCGTCACTGTCGAGCGAAAGGCACTCGCCTTCGTTCGCGACGTTACGAAAGTATGCCGCC
GCTTGTGGCAAGCGACTGGTGATCTCGTTTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References