Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4428324..4428943 Replicon chromosome
Accession NZ_CP055325
Organism Klebsiella michiganensis strain RHBSTW-00409

Toxin (Protein)


Gene name Hha Uniprot ID J5X5R4
Locus tag HV187_RS21230 Protein ID WP_004848333.1
Coordinates 4428725..4428943 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J4WQ74
Locus tag HV187_RS21225 Protein ID WP_009653563.1
Coordinates 4428324..4428698 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV187_RS21215 4423483..4424676 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV187_RS21220 4424699..4427845 + 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit -
HV187_RS21225 4428324..4428698 + 375 WP_009653563.1 Hha toxicity modulator TomB Antitoxin
HV187_RS21230 4428725..4428943 + 219 WP_004848333.1 hemolysin expression modulator Hha Toxin
HV187_RS21235 4429105..4429671 + 567 WP_004848331.1 maltose O-acetyltransferase -
HV187_RS21240 4429798..4430268 + 471 WP_177340516.1 YlaC family protein -
HV187_RS21245 4430243..4431697 - 1455 WP_049077911.1 PLP-dependent aminotransferase family protein -
HV187_RS21250 4431799..4432497 + 699 WP_128349260.1 GNAT family N-acetyltransferase -
HV187_RS21255 4432494..4432634 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HV187_RS21260 4432634..4432897 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8626.03 Da        Isoelectric Point: 8.9008

>T162341 WP_004848333.1 NZ_CP055325:4428725-4428943 [Klebsiella michiganensis]
MSDKTLTKVDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T162341 NZ_CP071746:1398545-1398796 [Burkholderia pseudomallei]
ATGCAGCGACTGCGCGATTTTCTGCGACCGAAGAATCCTGACGCCGCAAGGCGAGCTGGCGAAGCGATTAGACAAGGCGT
ACAGGCTCTCGGTACTCACCCTCGAATGGGGCGTCTCGTAGAAGATCTACCCGAGCAGTATCGGGAGTGGCCCATCGATT
TCGGGGACAGTGGTTACGTGGCCCGGTATCGCTTCGATGGCGACGCCGTCACGATCTTGGCTGTCCGTCATCAGAAGGAA
GCGGGGTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14378.14 Da        Isoelectric Point: 4.8989

>AT162341 WP_009653563.1 NZ_CP055325:4428324-4428698 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPVSLSC

Download         Length: 375 bp

>AT162341 NZ_CP071746:1398240-1398518 [Burkholderia pseudomallei]
ATGGTTACGGCAACATCCATCAAGCTTGATGATGAACTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCTCGTCGCCGCAC
CCCGCATTGGATCATGCGTGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGTATTGAACCAGGATACGC
TCAAGGCATGGGATGAATTTCAGGAGACTGGCCTGCACGCAACCGCCGAGGAGGTGGATAAGTGGCTTGCGAGCTGGGGA
ACTGAAAACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R4L0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R4I8

References