Detailed information of TA system TA162332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2108579..2109169
• Replicon: chromosome
• Accession: NZ_CP055325
• Organism: Klebsiella michiganensis strain RHBSTW-00409

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV187_RS10195
• Protein ID: WP_177339968.1
• Coordinates: 2108837..2109169 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: HV187_RS10190
• Protein ID: WP_004852307.1
• Coordinates: 2108579..2108836 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV187_RS10170	2104477..2105085	-	609	WP_057174013.1	glutathione S-transferase N-terminal domain-containing protein	-
HV187_RS10175	2105262..2106188	+	927	WP_014838892.1	LysR family transcriptional regulator	-
HV187_RS10180	2106226..2107224	-	999	WP_181248395.1	aldo/keto reductase	-
HV187_RS10185	2107325..2108239	+	915	WP_086531142.1	LysR family transcriptional regulator	-
HV187_RS10190	2108579..2108836	+	258	WP_004852307.1	antitoxin	Antitoxin
HV187_RS10195	2108837..2109169	+	333	WP_177339968.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV187_RS10205	2109492..2110949	+	1458	WP_032743138.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HV187_RS10215	2111913..2113367	-	1455	WP_004852297.1	AMP nucleosidase	-
HV187_RS10220	2113499..2113756	-	258	WP_177339969.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11837.72 Da
• Isoelectric Point: 10.5834

>T162332 WP_177339968.1 NZ_CP055325:2108837-2109169 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTTGIIRCDQPRTI
DIAARNGKRLERIPDAVVNDVLARLDAIFS

Nucleotide

• Length: 333 bp

>T162332 NZ_CP071734:817560-817970 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT162332 WP_004852307.1 NZ_CP055325:2108579-2108836 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT162332 NZ_CP071734:817313-817579 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8