Detailed information of TA system TA162327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1363930..1364537
• Replicon: chromosome
• Accession: NZ_CP055306
• Organism: Mannheimia pernigra strain BNO311

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HV559_RS06600
• Protein ID: WP_176809902.1
• Coordinates: 1363930..1364304 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HV559_RS06605
• Protein ID: WP_176809903.1
• Coordinates: 1364301..1364537 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV559_RS06590	1359041..1360948	-	1908	WP_176809900.1	hypothetical protein	-
HV559_RS06595	1360949..1363744	-	2796	WP_176809901.1	AAA family ATPase	-
HV559_RS06600	1363930..1364304	-	375	WP_176809902.1	endoribonuclease MazF	Toxin
HV559_RS06605	1364301..1364537	-	237	WP_176809903.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV559_RS06610	1364754..1365794	-	1041	WP_176809904.1	polysulfide reductase NrfD	-
HV559_RS06615	1365796..1366515	-	720	WP_176809905.1	tetrathionate reductase subunit TtrB	-
HV559_RS06620	1366742..1368682	+	1941	WP_176809906.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (6-50)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13914.25 Da
• Isoelectric Point: 9.4314

>T162327 WP_176809902.1 NZ_CP055306:c1364304-1363930 [Mannheimia pernigra]
MMSEYVPDVGDIIWLNFTPQVGHEQAGHRPAVVLTPASYNQLTGLLIYCPLTTKIKGYPFEVHIAGKPQNVVLSDQVKSL
DWRVRKAEFKGKIAESELMEIRKKVGVLLGITEPNHRFTKSALL

Nucleotide

• Length: 375 bp

>T162327 NZ_CP071733:555246-555530 [Bifidobacterium longum subsp. infantis]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAACACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATACGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGGCCCGGGGCATGACCCAAATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAAACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8640.85 Da
• Isoelectric Point: 4.3344

>AT162327 WP_176809903.1 NZ_CP055306:c1364537-1364301 [Mannheimia pernigra]
MQLAIKKWGNSVGVRIPASILTALQLQADDLVNIWAENGKIIIEPIRQEYSLEQLLSGITAENLHQEIETGESVGKEQ

Nucleotide

• Length: 237 bp

>AT162327 NZ_CP071733:554947-555249 [Bifidobacterium longum subsp. infantis]
ATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGCCCGGCT
CGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGTTCCACA
CCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAAAGCACC
CAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA