Detailed information of TA system TA162326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 255383..256023
• Replicon: chromosome
• Accession: NZ_CP055306
• Organism: Mannheimia pernigra strain BNO311

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: HV559_RS01150
• Protein ID: WP_176809344.1
• Coordinates: 255383..255787 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: W0Q267
• Locus tag: HV559_RS01155
• Protein ID: WP_025235144.1
• Coordinates: 255784..256023 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV559_RS01135	253846..254346	-	501	WP_176809341.1	hypothetical protein	-
HV559_RS01140	254437..254739	-	303	WP_176809342.1	hypothetical protein	-
HV559_RS01145	254948..255208	+	261	WP_176809343.1	helix-turn-helix domain-containing protein	-
HV559_RS01150	255383..255787	-	405	WP_176809344.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV559_RS01155	255784..256023	-	240	WP_025235144.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV559_RS01160	256278..256643	-	366	WP_005621166.1	50S ribosomal protein L7/L12	-
HV559_RS01165	256702..257193	-	492	WP_159628947.1	50S ribosomal protein L10	-
HV559_RS01170	257472..258161	-	690	WP_176807921.1	50S ribosomal protein L1	-
HV559_RS01175	258166..258594	-	429	WP_159628945.1	50S ribosomal protein L11	-
HV559_RS01180	258740..259303	-	564	WP_176807920.1	transcription termination/antitermination protein NusG	-
HV559_RS01185	259305..259718	-	414	WP_176807919.1	preprotein translocase subunit SecE	-
HV559_RS01190	259831..260850	-	1020	WP_176807918.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-116)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15121.67 Da
• Isoelectric Point: 7.6657

>T162326 WP_176809344.1 NZ_CP055306:c255787-255383 [Mannheimia pernigra]
MIYMLDTNILIYLMKNRPLAVAERVAQLTPSDQLVMSFITYAELLKGANGSANPEKALANIEKLKQRISVVYPNEKICEF
YGVWANKLKLQGKPIGGNDLWIACHALACNAILVTHNVKESERIEALNWQDWTC

Nucleotide

• Length: 405 bp

>T162326 NZ_CP071733:554947-555249 [Bifidobacterium longum subsp. infantis]
ATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGCCCGGCT
CGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGTTCCACA
CCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAAAGCACC
CAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9108.09 Da
• Isoelectric Point: 3.9281

>AT162326 WP_025235144.1 NZ_CP055306:c256023-255784 [Mannheimia pernigra]
MRTKVFQSGNSQAVRIPFDFRFDVDTVEILQGQNGDVILRPVKPQADMEFLALFSDFDDDFIQALEDRDTSPPQERDDL

Nucleotide

• Length: 240 bp

>AT162326 NZ_CP071733:555246-555530 [Bifidobacterium longum subsp. infantis]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAACACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATACGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGGCCCGGGGCATGACCCAAATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAAACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7D5HSX4