Detailed information of TA system TA162323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1425453..1426009
• Replicon: chromosome
• Accession: NZ_CP055305
• Organism: Mannheimia pernigra strain 17CN0883

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HV560_RS06885
• Protein ID: WP_176812491.1
• Coordinates: 1425453..1425773 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HV560_RS06890
• Protein ID: WP_176812492.1
• Coordinates: 1425770..1426009 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV560_RS06875	1422830..1424521	-	1692	WP_176812489.1	hypothetical protein	-
HV560_RS06880	1424518..1425456	-	939	WP_176812490.1	hypothetical protein	-
HV560_RS06885	1425453..1425773	-	321	WP_176812491.1	endoribonuclease MazF	Toxin
HV560_RS06890	1425770..1426009	-	240	WP_176812492.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV560_RS06895	1426330..1427622	+	1293	WP_176812493.1	ATP-binding protein	-
HV560_RS06900	1427637..1429592	+	1956	WP_176812494.1	DUF2075 domain-containing protein	-
HV560_RS06905	1429644..1430687	-	1044	WP_176812495.1	polysulfide reductase NrfD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1422830..1435858	13028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-101)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11801.86 Da
• Isoelectric Point: 8.4983

>T162323 WP_176812491.1 NZ_CP055305:c1425773-1425453 [Mannheimia pernigra]
MSEYVPDVGDIIWLNFTPQAGHRPVVVLTPASYNQLTSLLICCPLTTKIKGYPFEVHIAGKPQNVVLSDQVKSLDWRVRQ
AEFKGKIAESELVEIRKKVGVLLGLV

Nucleotide

• Length: 321 bp

>T162323 NZ_CP071733:c87711-87355 [Bifidobacterium longum subsp. infantis]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTACGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTCCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCAGCGTCGCCTG
GGAGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8748.05 Da
• Isoelectric Point: 4.5125

>AT162323 WP_176812492.1 NZ_CP055305:c1426009-1425770 [Mannheimia pernigra]
MQLAIKKWGNSVGVRIPASILTALQLQADNLVDIRAENGKIIIEPIRQEYSLEQLLSGITAENLHQEIETGEPIGKEQL

Nucleotide

• Length: 240 bp

>AT162323 NZ_CP071733:c87994-87698 [Bifidobacterium longum subsp. infantis]
ATGATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCAT
GGGGTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCG
TCCGCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCC
CAGATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA