Detailed information of TA system TA162322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 644030..644670
• Replicon: chromosome
• Accession: NZ_CP055305
• Organism: Mannheimia pernigra strain 17CN0883

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: HV560_RS03190
• Protein ID: WP_176809344.1
• Coordinates: 644266..644670 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: W0Q267
• Locus tag: HV560_RS03185
• Protein ID: WP_025235144.1
• Coordinates: 644030..644269 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV560_RS03150	639072..640211	+	1140	WP_176812130.1	DNA-processing protein DprA	-
HV560_RS03155	640334..640747	+	414	WP_176807919.1	preprotein translocase subunit SecE	-
HV560_RS03160	640749..641312	+	564	WP_176807920.1	transcription termination/antitermination protein NusG	-
HV560_RS03165	641458..641886	+	429	WP_159628945.1	50S ribosomal protein L11	-
HV560_RS03170	641891..642580	+	690	WP_176807921.1	50S ribosomal protein L1	-
HV560_RS03175	642860..643351	+	492	WP_159628947.1	50S ribosomal protein L10	-
HV560_RS03180	643410..643775	+	366	WP_005621166.1	50S ribosomal protein L7/L12	-
HV560_RS03185	644030..644269	+	240	WP_025235144.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV560_RS03190	644266..644670	+	405	WP_176809344.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV560_RS03195	644847..645107	-	261	WP_176807923.1	helix-turn-helix domain-containing protein	-
HV560_RS03200	645322..645759	+	438	WP_176812131.1	hypothetical protein	-
HV560_RS03205	645774..646076	+	303	WP_176812132.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-116)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15121.67 Da
• Isoelectric Point: 7.6657

>T162322 WP_176809344.1 NZ_CP055305:644266-644670 [Mannheimia pernigra]
MIYMLDTNILIYLMKNRPLAVAERVAQLTPSDQLVMSFITYAELLKGANGSANPEKALANIEKLKQRISVVYPNEKICEF
YGVWANKLKLQGKPIGGNDLWIACHALACNAILVTHNVKESERIEALNWQDWTC

Nucleotide

• Length: 405 bp

>T162322 NZ_CP071733:c35665-35342 [Bifidobacterium longum subsp. infantis]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACAGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9108.09 Da
• Isoelectric Point: 3.9281

>AT162322 WP_025235144.1 NZ_CP055305:644030-644269 [Mannheimia pernigra]
MRTKVFQSGNSQAVRIPFDFRFDVDTVEILQGQNGDVILRPVKPQADMEFLALFSDFDDDFIQALEDRDTSPPQERDDL

Nucleotide

• Length: 240 bp

>AT162322 NZ_CP071733:c35897-35652 [Bifidobacterium longum subsp. infantis]
ATGAGCATACAGATTGCCACGCGAGTTAGTGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7D5HSX4