Detailed information of TA system TA162321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 230519..231150
• Replicon: chromosome
• Accession: NZ_CP055305
• Organism: Mannheimia pernigra strain 17CN0883

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV560_RS01055
• Protein ID: WP_176807449.1
• Coordinates: 230519..230917 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HV560_RS01060
• Protein ID: WP_176807450.1
• Coordinates: 230917..231150 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV560_RS01035	227352..227618	-	267	WP_176807446.1	chaperone NapD	-
HV560_RS01040	227635..228159	-	525	WP_176810358.1	ferredoxin-type protein NapF	-
HV560_RS01045	228328..228903	+	576	WP_176811971.1	NAD(P)H-dependent oxidoreductase	-
HV560_RS01050	228964..230499	-	1536	WP_176811972.1	L-2,4-diaminobutyrate decarboxylase	-
HV560_RS01055	230519..230917	-	399	WP_176807449.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HV560_RS01060	230917..231150	-	234	WP_176807450.1	antitoxin	Antitoxin
HV560_RS01065	231319..232683	-	1365	WP_176811973.1	diaminobutyrate--2-oxoglutarate transaminase	-
HV560_RS01070	233031..233201	-	171	WP_159628615.1	50S ribosomal protein L33	-
HV560_RS01075	233213..233449	-	237	WP_006251446.1	50S ribosomal protein L28	-
HV560_RS01080	233620..234312	-	693	WP_176807453.1	DNA repair protein RadC	-
HV560_RS01085	234470..235678	+	1209	WP_176812813.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
HV560_RS01090	235675..235992	+	318	WP_176809332.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14891.17 Da
• Isoelectric Point: 8.0586

>T162321 WP_176807449.1 NZ_CP055305:c230917-230519 [Mannheimia pernigra]
MLKYMLDSNIVIYVIKHRPVEVLQRFNQNAGKMCVSSITAAELYYGAEKSGFSARNIAVIEDFLSRLTILDYQPKSAAHF
GNIKAELAKQGKLIGENDIHIAAHARSEGLVIVTNNLREFERVEGLRLENWV

Nucleotide

• Length: 399 bp

>T162321 NZ_CP071731:6253181-6253759 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9009.22 Da
• Isoelectric Point: 4.3094

>AT162321 WP_176807450.1 NZ_CP055305:c231150-230917 [Mannheimia pernigra]
MIEASIFMTNRSQAVRLPAEVRFDNDVKKIAVRILGNERILSPLNQSWDSFFLNSETVSEDFMVEREIAMQPEREDF

Nucleotide

• Length: 234 bp

>AT162321 NZ_CP071731:6252538-6253092 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA