Detailed information of TA system TA162317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1582379..1582973
• Replicon: chromosome
• Accession: NZ_CP055302
• Organism: Mannheimia pernigra strain 16CN0041

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HV561_RS08130
• Protein ID: WP_176809025.1
• Coordinates: 1582815..1582973 (-)
• Length: 53 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV561_RS08125
• Protein ID: WP_176808486.1
• Coordinates: 1582379..1582792 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV561_RS08085	1578468..1579010	-	543	WP_176808478.1	phage tail protein I	-
HV561_RS08090	1579000..1579917	-	918	WP_176808479.1	baseplate J/gp47 family protein	-
HV561_RS08095	1579914..1580249	-	336	WP_176808480.1	GPW/gp25 family protein	-
HV561_RS08100	1580249..1580818	-	570	WP_176808481.1	phage baseplate assembly protein V	-
HV561_RS08105	1580945..1581232	-	288	WP_176808482.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV561_RS08110	1581225..1581404	-	180	WP_176808483.1	hypothetical protein	-
HV561_RS08115	1581544..1581975	-	432	WP_176808484.1	type II toxin-antitoxin system HicB family antitoxin	-
HV561_RS08120	1582036..1582209	-	174	WP_176808485.1	mRNA interferase	-
HV561_RS08125	1582379..1582792	-	414	WP_176808486.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV561_RS08130	1582815..1582973	-	159	WP_176809025.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV561_RS08135	1583143..1586121	-	2979	WP_176808487.1	phage tail tape measure protein	-
HV561_RS08140	1586158..1586409	+	252	WP_176808488.1	hypothetical protein	-
HV561_RS08145	1586524..1586802	+	279	WP_176808489.1	hypothetical protein	-
HV561_RS08150	1586830..1587294	-	465	WP_176808490.1	phage virion morphogenesis protein	-
HV561_RS08155	1587287..1587772	-	486	WP_176808491.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1550059..1595729	45670

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-51)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-130)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5791.64 Da
• Isoelectric Point: 10.6715

>T162317 WP_176809025.1 NZ_CP055302:c1582973-1582815 [Mannheimia pernigra]
MIEADGWVFHHATGSHHHFTHPTKKGVVTVPHPRKDVKKGTENSILKQAGLK

Nucleotide

• Length: 159 bp

>T162317 NZ_CP071731:c1298436-1298137 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15135.33 Da
• Isoelectric Point: 4.4776

>AT162317 WP_176808486.1 NZ_CP055302:c1582792-1582379 [Mannheimia pernigra]
MLYPIAIEPGDETHAFGVIVPDIPGCFSAGDTLEEAYANAKEAIKGHLELLVEMGEEVPLPTSIDNHRKNPDFTEYDMFF
GVVDVDITHLLGKAEKINITMPAYLIKRIDEFVATHKEYKSRSGFLAQIAADKILAA

Nucleotide

• Length: 414 bp

>AT162317 NZ_CP071731:c1298140-1297850 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA